Dietary calcium supplementation promotes the accumulation of intramuscular fat

Autor:	Tingli Pan, Ziyi Song, Zhiwang Zhang, Haojie Zhang, Siqi Liu, Lei Zhou, Yixing Li, Yu Sun
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	medicine.medical_specialty Veterinary medicine chemistry.chemical_element Calcium Biochemistry SF1-1100 chemistry.chemical_compound Gastrocnemius muscle Internal medicine SF600-1100 medicine Glycolysis Meat quality Pig Triglyceride Research Lipid metabolism Intramuscular fat musculoskeletal system Animal culture Endocrinology Calcium carbonate chemistry Animal Science and Zoology C2C12 Increased intramuscular fat Food Science Biotechnology
Zdroj:	Journal of Animal Science and Biotechnology, Vol 12, Iss 1, Pp 1-15 (2021) Journal of Animal Science and Biotechnology
ISSN:	2049-1891
Popis:	Background In the livestock industry, intramuscular fat content is a key factor affecting meat quality. Many studies have shown that dietary calcium supplementation is closely related to lipid metabolism. However, few studies have examined the relationship between dietary calcium supplementation and intramuscular fat accumulation. Methods Here, we used C2C12 cells, C57BL/6 mice (n = 8) and three-way cross-breeding pigs (Duroc×Landrace×Large white) (n = 10) to study the effect of calcium addition on intramuscular fat accumulation. In vitro, we used calcium chloride to adjust the calcium levels in the medium (2 mmol/L or 3 mmol/L). Then we measured various indicators. In vivo, calcium carbonate was used to regulate calcium levels in feeds (Mice: 0.5% calcium or 1.2% calcium) (Pigs: 0.9% calcium or 1.5% calcium). Then we tested the mice gastrocnemius muscle triglyceride content, pig longissimus dorsi muscle meat quality and lipidomics. Results In vitro, calcium addition (3 mmol/L) had no significant effect on cell proliferation, but promoted the differentiation of C2C12 cells into slow-twitch fibers. Calcium supplementation increased triglyceride accumulation in C2C12 cells. Calcium addition increased the number of mitochondria and also increased the calcium level in the mitochondria and reduced the of key enzymes activity involved in β-oxidation such as acyl-coenzyme A dehydrogenase. Decreasing mitochondrial calcium level can alleviate lipid accumulation induced by calcium addition. In addition, calcium addition also reduced the glycolytic capacity and glycolytic conversion rate of C2C12 cells. In vivo, dietary calcium supplementation (1.2%) promoted the accumulation of triglycerides in the gastrocnemius muscle of mice. Dietary calcium supplementation (1.5%) had no effect on pig weight, but significantly improved the flesh color of the longissimus dorsi muscle, reduced the backfat thickness and increased intramuscular fat content in pigs. Besides, calcium addition had no effect on longissimus dorsi pH, electrical conductivity and shear force. Conclusions These results suggest that calcium addition promotes intramuscular fat accumulation by inhibiting the oxidation of fatty acids. These findings provide a new tool for increasing intramuscular fat content and an economical strategy for improving meat quality.
Databáze:	OpenAIRE
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