Micropropagation of Cleome dendroides (Cleomaceae), an endemic Brazilian species, as a source of glucosinolates
| Autor: | Marsen Garcia Pinto Coelho, Claudia Simões-Gurgel, Tatiana Carvalho de Castro, Norma Albarello, Carlos Roberto Machado Gayer |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences biology fungi Biome food and beverages Plant Science biology.organism_classification Cleome 01 natural sciences 03 medical and health sciences Micropropagation Phytochemical Botany Cleomaceae Atlantic forest Endemism Ecology Evolution Behavior and Systematics 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 155:281-290 |
| ISSN: | 1724-5575 1126-3504 |
| DOI: | 10.1080/11263504.2020.1739161 |
| Popis: | This is the first study related to micropropagation and phytochemical analyses of Cleome dendroides, an endemic species of the Brazilian Atlantic Forest, a biome affected by human activity. Among the classes of substances produced by the genus Cleome, the glucosinolates are used as chemotaxonomic markers. They have a wide structural diversity, which affords, for example, antineoplastic bioactivity. The micropropagation was established using explants from two sources: seedlings and in vitro plants. The morphogenic response was evaluated on Murashige-Skoog (MS) medium with different concentrations of benzyladenine (BA). Hypocotyl explants from seedlings and stem explants from in vitro plants showed higher capacity of shoot regeneration (56.3-100%). Shoot proliferation (75-100%) was maintained when hypocotyl explant-derived shoots and stem explant-derived shoots were subcultured three times a month. Hypocotyl explant-derived shoots maintained on MS + 2.0 mgL−1 BA induced the highest shoot production along the subcultures. Shoots were elongated and rooted on growth regulator-free MS medium. Plantlet establishment was successful in the hardening medium composed of Plantmax® substrate, reaching 85%survival. The phytochemical studies indicated two glucosinolate derivatives, highlighting the presence of 5,6-dimethyltetrahydro-1,3-oxazine-2-thione, identified for the first time in plants. This work demonstrates an efficient method for in-vitro production of C. dendroides plants, which can be used as a source of glucosinolates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|