Generation of a human iPS cell line (CABi003-A) from a patient with age-related macular degeneration carrying the CFH Y402H polymorphism
| Autor: | Ana B. García-Delgado, Adoración Montero-Sánchez, Beatriz Ponte-Zuñiga, Berta de la Cerda, Sofia Calado, Lourdes Valdés-Sánchez, S. S. Bhattacharya, Francisco J. Diaz-Corrales |
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| Přispěvatelé: | Instituto de Salud Carlos III, European Commission, Universidad de Sevilla. Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Sevilla. Departamento de Cirugía, Instituto de Salud Carlos III (ISCIII). España, European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty genetic structures Immunocytochemistry Induced Pluripotent Stem Cells Peripheral blood mononuclear cell Cell Line 03 medical and health sciences Macular Degeneration 0302 clinical medicine medicine Humans Cellular Reprogramming Techniques Induced pluripotent stem cell lcsh:QH301-705.5 Aged 80 and over Retina Polymorphism Genetic biology Cell Biology General Medicine Macular degeneration medicine.disease biology.organism_classification Sendai virus eye diseases 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Complement Factor H Female sense organs Reprogramming 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Stem Cell Research, Vol 38, Iss, Pp-(2019) |
| Popis: | Age-related macular degeneration (AMD) is the leading cause of adult blindness in developed countries and is characterized by progressive degeneration of the macula, the central region of the retina. A human induced pluripotent stem cell (hiPSC) line was derived from peripheral blood mononuclear cells (PBMCs) from a patient with a clinical diagnosis of dry AMD carrying the CFH Y402H polymorphism. Sendai virus was using for reprogramming and the pluripotent and differentiation capacity of the cells were assessed by immunocytochemistry and RT-PCR. This work was supported by ISCIII, Spain (Miguel Servet-I, 2015) and cofinanced by European Regional Development Fund (ERDF) (CP 15/00071). |
| Databáze: | OpenAIRE |
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