Clinical application of a cytochemical bioassay for the determination of thyroid stimulating hormone
| Autor: | T. Hashimoto, A. von zur Mühlen, K.-D. Döhler, Dieter Emrich |
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| Rok vydání: | 1979 |
| Předmět: |
Male
endocrine system medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism Graves' disease Guinea Pigs Radioimmunoassay Thyrotropin Stimulation Iodine Radioisotopes Basal (phylogenetics) Endocrinology TRH stimulation test Thyroid-stimulating hormone Internal medicine medicine Animals Humans Thyrotropin-Releasing Hormone Aged Goiter Histocytochemistry business.industry Thyroid Middle Aged medicine.disease Graves Disease 3. Good health medicine.anatomical_structure Thyroidectomy Female business hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Journal of Endocrinological Investigation. 2:395-400 |
| ISSN: | 1720-8386 0391-4097 |
| DOI: | 10.1007/bf03349339 |
| Popis: | Thyroid stimulating hormone (TSH) was measured using a highly sensitive cytochemical bioassay (CBA) technique in normal subjects, in patients with Graves' disease (untreated and treated) and in patients with euthyroid goiter and negative thyrotropin-releasing hormone (TRH)-test. Plasma TSH levels of normal subjects and of subjects with untreated Graves' disease were reduced after the plasma had been incubated with a specific antibody to human TSH, indicating that the thyroid stimulating substance measured with this assay was likely to be TSH. Basal TSH levels in patients with Graves' disease and in a special group of patients with euthyroid goiter and negative TRH test were low but detectable. They did not rise after TRH administration. Increased TSH release (4.5- to 10-fold) after TRH stimulation was demonstrated, however, in two formerly hyperthyroid patients after treatment. This increase was not detected by radioimmunoassay (RIA) due to limited sensitivity. |
| Databáze: | OpenAIRE |
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