Virus-induced synthesis of messenger RNAs for precursors of pathogenesis-related proteins in tobacco
| Autor: | J. H. Van Boom, R. A. M. Hooft van Huijsduijnen, M. Tromp, Ben J. C. Cornelissen, John F. Bol, L.C. van Loon |
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| Rok vydání: | 1985 |
| Předmět: |
Signal peptide
DNA Complementary Base Sequence General Immunology and Microbiology biology General Neuroscience Nicotiana tabacum Tobamovirus Articles Blotting Northern biology.organism_classification Molecular biology General Biochemistry Genetics and Molecular Biology Virus Tobacco Mosaic Virus Complementary DNA Tobacco Tobacco mosaic virus Immunoprecipitation Amino Acid Sequence RNA Messenger Northern blot Molecular Biology Peptide sequence Plant Proteins |
| Zdroj: | The EMBO Journal. 4:2167-2171 |
| ISSN: | 0261-4189 |
| DOI: | 10.1002/j.1460-2075.1985.tb03911.x |
| Popis: | Infection of Samsun NN tobacco with tobacco mosaic virus (TMV) induces a number of host-encoded, so-called pathogenesis-related (PR-) proteins, which are found in the intercellular space of the leaf and are associated with induced resistance. By immunoprecipitation of their in vitro translation products we were able to detect the mRNAs corresponding to a number of PR-proteins in TMV-infected tobacco, but not in healthy plants. Analysis by the Northern blot technique using cloned cDNA of PR1-mRNAs as probe showed that the mRNAs for the closely related proteins PR1a, 1b and 1c occur at a low level in healthy tobacco; upon TMV infection this level is increased >100-fold. The PR1-specific probe did not hybridize to mRNAs corresponding to other PR-proteins. Sequencing of the 5'-terminal region of PR1-mRNAs showed that PR1-proteins are derived from precursors by removal of an N-terminal signal peptide of 30 amino acids. |
| Databáze: | OpenAIRE |
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