Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis
| Autor: | Alana Lucena Oliveira, Ana Carolina Chipoletti Prado, Giselle Souza da Paz, Jéssica Luana Chechi, José Cavalcante Souza Vieira, Anderson Messias Rodrigues, Tiwa Rotchanapreeda, Sandra de Moraes Gimenes Bosco, Lucilene Delazari dos Santos, Marília Afonso Rabelo Buzalaf, Theerapong Krajaejun |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Mahidol University, Universidade de São Paulo (USP) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
QH301-705.5 diagnosis Pythium insidiosum Plant Science Disease Immunoproteomics Article Microbiology 03 medical and health sciences Pythiosis Immune system Antigen antigens Diagnosis Biology (General) Antigens Pathogen Ecology Evolution Behavior and Systematics 030304 developmental biology Oomycete 0303 health sciences therapy biology 030306 microbiology PROTEÔMICA pythiosis biology.organism_classification immunoproteomics Therapy |
| Zdroj: | Journal of Fungi Volume 7 Issue 6 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Journal of Fungi, Vol 7, Iss 423, p 423 (2021) |
| ISSN: | 2309-608X |
| Popis: | Made available in DSpace on 2021-06-25T11:02:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-06-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Pythiosis, whose etiological agent is the oomycete Pythium insidiosum, is a life-threatening disease that occurs mainly in tropical and subtropical countries, affecting several animal species. It is frequently found in horses in Brazil and humans in Thailand. The disease is difficult to diagnose because the pathogen’s hyphae are often misdiagnosed as mucoromycete fungi in histological sections. Additionally, there is no specific antigen to use for rapid diagnosis, the availability of which could improve the prognosis in different animal species. In this scenario, we investigated which P. insidiosum antigens are recognized by circulating antibodies in horses and humans with pythiosis from Brazil and Thailand, respectively, using 2D immunoblotting followed by mass spectrometry for the identification of antigens. We identified 23 protein spots, 14 recognized by pooled serum from horses and humans. Seven antigens were commonly recognized by both species, such as the heat-shock cognate 70 KDa protein, the heat-shock 70 KDa protein, glucan 1,3-beta-glucosidase, fructose-bisphosphate aldolase, serine/threonine-protein phosphatase, aconitate hydratase, and 14-3-3 protein epsilon. These results demonstrate that there are common antigens recognized by the immune responses of horses and humans, and these antigens may be studied as biomarkers for improving diagnosis and treatment. Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP) Department of Pathology Faculty of Medicine Ramathibodi Hospital Mahidol University Faculty of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP) Department of Biological Sciences Bauru School of Dentistry University of São Paulo (USP) Department of Microbiology Immunology and Parasitology Cell Biology Division Federal University of São Paulo (UNIFESP) Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP) Graduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP) Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP) Faculty of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP) Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP) Graduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP) CAPES: 001 FAPESP: 2016/10804-8 FAPESP: 2018/08009-0 FAPESP: 2019/08761-7 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|