Identification of a bone marrow–derived epithelial-like population capable of repopulating injured mouse airway epithelium
| Autor: | Xing-Hua Wang, Adrian G. Sacher, Thomas K. Waddell, Armand Keating, Wei-Yang Lu, Amy P. Wong, Pascal Duchesneau, Jim Hu |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty Cellular differentiation Population Cystic Fibrosis Transmembrane Conductance Regulator Bone Marrow Cells Receptors Cell Surface Lung injury Biology Flow cytometry Mice Antigens CD medicine Animals Humans Regeneration Cell Lineage Progenitor cell education education.field_of_study medicine.diagnostic_test Endoglin Cell Differentiation Epithelial Cells General Medicine Lung Injury respiratory system Epithelium Mice Inbred C57BL medicine.anatomical_structure Respiratory epithelium Leukocyte Common Antigens Bone marrow Research Article |
| Popis: | The bone marrow compartment is enriched in stem and progenitor cells, and an unidentified subpopulation of these cells can contribute to lung epithelial repair. Here we identify this subpopulation and quantitate its relative contribution to injured airway epithelium. A subpopulation of adherent human and murine bone marrow cells that expresses Clara cell secretory protein (CCSP) was identified using flow cytometry. When cultured at the air-liquid interface in ex vivo cultures, Ccsp+ cells expressed type I and type II alveolar markers as well as basal cell markers and active epithelial sodium channels. Ccsp+ cells preferentially homed to naphthalene-damaged airways when delivered transtracheally or intravenously, with the former being more efficient than the latter. Interestingly, naphthalene-induced lung damage transiently increased Ccsp expression in bone marrow and peripheral circulation. Furthermore, lethally irradiated Ccsp-null mice that received tagged wild-type bone marrow contained donor-derived epithelium in both normal and naphthalene-damaged airways. This study therefore identifies what we believe to be a newly discovered cell in the bone marrow that might have airway reconstitution potential in the context of cell-based therapies for lung disease. Additionally, these data could reconcile previous controversies regarding the contribution of bone marrow to lung regeneration. |
| Databáze: | OpenAIRE |
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