Ovarian follicular differentiation with prepubertal gonadotropin surges and gonadotropin priming in mice
| Autor: | Kazumichi Andoh, Xiaowei Liu, Hua Wang, Kiyohiko Yamada, Fatima Riasat, Junro Kobayashi, Yoshito Ibuki, Yumiko Abe, Hideki Mizunuma, Hidemi Yokota, Takanori Kamijo, Nobumasa Kikuchi |
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| Rok vydání: | 2002 |
| Předmět: |
Aging
endocrine system medicine.medical_specialty medicine.drug_class Radioimmunoassay Priming (immunology) Biology General Biochemistry Genetics and Molecular Biology Human chorionic gonadotropin Gonadotropin-Releasing Hormone Mice Organ Culture Techniques Ovarian Follicle Internal medicine Follicular phase medicine Animals Inhibins Sexual Maturation General Pharmacology Toxicology and Pharmaceutics Estradiol General Medicine In vitro Activin a Endocrinology Female Follicle Stimulating Hormone Gonadotropin Luteinizing hormone Gonadotropins hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Life Sciences. 71:1187-1197 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(02)01829-5 |
| Popis: | Preantral follicles were mechanically isolated from the ovaries of 1.5 to 8 week old mice and cultured in vitro for 4 days in the presence or absence of either activin A or FSH. Plasma gonadotropin, estradiol and immunoreactive (IR) inhibin levels were measured. Cultured follicles showed stepwise changes in response to recombinant human (rh) FSH, with no response until 11 days, a gradual increase from 2 weeks, culminating in a strong response to rhFSH at 8 weeks. The response to activin A was vice versa. It enhanced the effect of rhFSH on preantral follicular growth of up to 4-week-old mice, but inhibited the effect of rhFSH in 8-week-old mice. The peak of the prepubertal gonadotropin surge was observed on day 11. Seven-day-old mice were treated with either luteinizing hormone releasing hormone (LHRH) or rhFSH or human chorionic gonadotropin (hCG) for 3 consecutive days from day 7, and follicles were collected on day 11. Those follicles showed enhanced response to rhFSH, no response to activin A, and an enhanced response to the combination of rhFSH and activin A, suggesting that the chronological changes in follicular response are a result of the prepubertal gonadotropin surge. |
| Databáze: | OpenAIRE |
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