Effects of 3 diets with various calcium contents on 24-h energy expenditure, fat oxidation, and adipose tissue message RNA expression of lipid metabolism-related proteins
| Autor: | Audrey Sicard, Gabby B. Hul, Wim H. M. Saris, Niels Boon, Nathalie Viguerie, Dominique Langin |
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| Přispěvatelé: | Humane Biologie, RS: NUTRIM School of Nutrition and Translational Research in Metabolism |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Adult
Male medicine.medical_specialty Peroxisome proliferator-activated receptor gamma Medicine (miscellaneous) Adipose tissue chemistry.chemical_element Calcium Biology Gene Expression Regulation Enzymologic Oxygen Consumption Calcitriol Internal medicine Gene expression medicine Humans RNA Messenger chemistry.chemical_classification Cross-Over Studies Nutrition and Dietetics Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Calorimetry Indirect Lipid metabolism Metabolism Lipid Metabolism Lipids Calcium Dietary Dose–response relationship Enzyme Endocrinology Adipose Tissue chemistry Body Composition Dairy Products Energy Metabolism Oxidation-Reduction |
| Zdroj: | Europe PubMed Central ResearcherID American Journal of Clinical Nutrition, 82(6), 1244-1252. Oxford University Press |
| ISSN: | 0002-9165 |
| Popis: | BACKGROUND: Evidence from molecular and animal research and epidemiologic investigations indicates that calcium intake may be inversely related to body weight, possibly through alterations in 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] metabolism. OBJECTIVE: We tested whether energy and substrate metabolism and adipose tissue enzyme messenger RNA (mRNA) expression can be altered by dietary calcium intake in healthy, nonobese, human volunteers consuming an isocaloric diet. DESIGN: Twelve healthy men [age: 28 +/- 2 y; body mass index (BMI; in kg/m(2)): 25.2 +/- 06] received 3 isocaloric diets [high calcium (1259 +/- 9 mg/d), high dairy (high/high); high calcium (1259 +/- 9 mg/d), low dairy (high/low); and low calcium (349 +/- 8 mg/d), low dairy (low/low)] in a randomized crossover design. At the end of the 7-d dietary periods, 24-h energy expenditure and substrate metabolism were measured, and fat biopsy specimens were obtained to determine mRNA expression in genes involved in the lipolytic and lipogenic pathways. RESULTS: The 24-h energy expenditure was 11.8 +/- 0.3, 11.6 +/- 0.3, and 11.7 +/- 0.3 MJ/24 h in the high/high, high/low, and low/low conditions, respectively. Fat oxidation in these conditions was 108 +/- 7, 105 +/- 9, and 100 +/- 6 g/24 h. These differences were not statistically significant. mRNA concentrations of UCP2, FAS, GPDH2, HSL, and PPARG did not differ significantly. Serum 1,25(OH)(2)D(3) concentrations changed from 175 +/- 16 to 138 +/- 15, 181 +/- 23 to 159 +/- 19, and 164 +/- 13 to 198 +/- 19 pmol/L in the high/high, high/low, and low/low conditions, respectively, and was significantly different between the high/high and low/low conditions (P < 0.05). CONCLUSION: Altering the dietary calcium content for 7 d does not influence substrate metabolism, energy metabolism, or gene expression in proteins related to fat metabolism, despite significant changes in 1,25(OH)(2)D(3) concentrations. |
| Databáze: | OpenAIRE |
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