Antagonism by reactive blue 2 but not by brilliant blue G of extracellular ATP-evoked responses in PC12 phaeochromocytoma cells
| Autor: | Ken Nakazawa, Tomoko Obama, Mica Ohara-Imaizumi, Kannosuke Fujimori, Akira Takanaka, Kazuhide Inoue |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Dopamine Adrenal Gland Neoplasms chemistry.chemical_element Pheochromocytoma Biology Calcium Membrane Potentials Dopamine secretion Adenosine Triphosphate Catecholamines Internal medicine Tumor Cells Cultured Extracellular medicine Protein Synthesis Inhibitors Pharmacology Membrane potential Triazines Benzenesulfonates Purinergic receptor Receptors Purinergic Antagonist Electrophysiology Endocrinology chemistry Cell culture Biophysics Antagonism Research Article |
| Zdroj: | British Journal of Pharmacology. 102:851-854 |
| ISSN: | 0007-1188 |
| DOI: | 10.1111/j.1476-5381.1991.tb12265.x |
| Popis: | 1. The effects of reactive blue 2 and brilliant blue G, which have been shown to block extracellular ATP-evoked responses, were investigated to discover whether these compounds act as P2-purinoceptor antagonists in PC12 phaeochromocytoma cells. 2. Reactive blue 2 (10 to 100 microM) suppressed the ATP-stimulated dopamine secretion from PC12 cells in a dose-dependent manner. The concentration-response curve for ATP was shifted to the right and the maximal response was decreased by reactive blue (30 and 100 microM). Brilliant blue G (up to 100 microM) did not significantly affect the secretion. 3. Reactive blue 2 (10 to 100 microM) suppressed the ATP-activated inward current recorded from the voltage-clamped cells in a concentration-dependent manner. Brilliant blue G (up to 100 microM) did not affect the current. 4. The results suggest that reactive blue 2 but not brilliant blue G is a P2-purinoceptor antagonist in PC12 cells. The purinoceptors in these cells may be the same type as those involved in ATP-evoked smooth muscle relaxation, judging from the antagonism by reactive blue 2. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text