Validated spectrophotometric determination of maduramicin ammonium using three charge transfer complexation reactions
| Autor: | Magdi M. Abdel-Khalek, Dina S. El-Kafrawy, Tarek S. Belal, Mostafa M. Baker |
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| Rok vydání: | 2020 |
| Předmět: |
Quality Control
Cost-Benefit Analysis Pharmaceutical Science Picric acid Pharmaceutical formulation 01 natural sciences Maduramicin ammonium Lactones chemistry.chemical_compound Limit of Detection Ammonium Compounds Pharmacology Detection limit chemistry.chemical_classification Chromatography 010405 organic chemistry 010401 analytical chemistry Reproducibility of Results Veterinary Drugs Electron acceptor Chromophore Charge-transfer complex 0104 chemical sciences chemistry Determination methods Indicators and Reagents Spectrophotometry Ultraviolet Powders |
| Zdroj: | Annales Pharmaceutiques Françaises. 78:388-397 |
| ISSN: | 0003-4509 |
| DOI: | 10.1016/j.pharma.2020.04.002 |
| Popis: | Summary Direct spectrophotometric determination of Maduramicin ammonium (MAD) represents an analytical challenge since it is a weak UV-absorbing and lacking a strong chromophore. This work represents the first spectrophotometric determination of MAD as no direct spectrophotometric or colorimetric determination methods for MAD are available in the literature. The present study illustrates the development of three simple, rapid and inexpensive colorimetric methods for the routine quality control analysis of MAD based on the formation of colored charge transfer complexes with three electron acceptors namely p-chloranilic acid (p-CA), 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) and picric acid (PA). The color products of MAD with p-CA, DDQ and PA were measured at 519, 588 and 405 nm respectively. The proposed methods were validated in terms of linearity, ranges, precision, accuracy, robustness and limits of detection and quantification. MAD was effectively determined over concentration ranges of 100–1000, 25–250 and 30–150 μg/mL using p-CA, DDQ and PA, respectively with good linearity as shown by the values of correlation coefficients not less than 0.9991. The developed methods were successfully implemented in the assay of MAD powder pharmaceutical formulation for veterinary use. |
| Databáze: | OpenAIRE |
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