Canola and hydrogenated soybean oils accelerate ectopic bone formation induced by implantation of bone morphogenetic protein in mice
| Autor: | Naoki Ohara, Tatsushi Kawai, Yoko Hashimoto, Akira Hanya, Shuichiro Kobayashi, Harumi Okuyama, Toshihide Noguchi, Mayumi Mori, Shin-ichi Watanabe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Vitamin K
Health Toxicology and Mutagenesis Hydrogenated soybean oil Toxicology Soybean oil Ectopic calcification Matrix gla protein TRI/3D-BON three-dimensional reconstruction imaging for bone Vitamin K2 (PubChem CID Canola dihydro-VK1 2′ 3′-dihydro-vitamin K1 Bone mineral Vitamin K1 (PubChem CID: 52384607) Kidney biology 4056): Vitamin K3 (PubChem CID: 4055) Chemistry food and beverages Dihydro-vitamin K1 (PubChem CID: 152059) ucMGP undercarboxylated MGP medicine.anatomical_structure Biochemistry Osteocalcin medicine.medical_specialty food.ingredient Gla-OC carboxylated osteocalcin Vitamin K1 hydroquinone (PubChem CID: 5280585) education Canola oil Bone morphogenetic protein Article VK vitamin K food G6PDH glucose-6-phosphate dehydrogenase lcsh:RA1190-1270 Internal medicine H2-Soy hydrogenated soybean oil SHRSP rat stroke-prone spontaneously hypertensive rat medicine Matrix Gla protein Soy soybean oil cMGP carboxylated matrix Gla protein lcsh:Toxicology. Poisons Gla carboxyglutamic acid fungi BMP bone morphogenetic protein medicine.disease Can canola oil Glu-OC undercarboxylated osteocalcin Endocrinology biology.protein mCT micro-computed tomography |
| Zdroj: | Toxicology Reports, Vol 1, Iss C, Pp 955-962 (2014) Toxicology Reports |
| ISSN: | 2214-7500 |
| Popis: | Highlights • BMP-induced bone formation was evaluated in mice fed a Soy, Can, or H2-Soy diet. • Ectopic bone formation was greater in the Can and H2-Soy groups. • Altered levels of VK2 and VK-dependent protein(s) might affect the bone formation. Canola oil (Can) and hydrogenated soybean oil (H2-Soy) are commonly used edible oils. However, in contrast to soybean oil (Soy), they shorten the survival of stroke-prone spontaneously hypertensive (SHRSP) rats. It has been proposed that the adverse effects of these oils on the kidney and testis are caused at least in part by dihydro-vitamin K (VK) 1 in H2-Soy and unidentified component(s) in Can. Increased intake of dihydro-VK1 is associated with decreased tissue VK2 levels and bone mineral density in rats and humans, respectively. The aim of the present study was to determine the effects of these oils on bone morphogenetic protein (BMP)-induced ectopic bone formation, which is promoted by VK2 deficiency, in relation to the role of VK in the γ-carboxylation of osteocalcin and matrix Gla protein. A crude extract of BMPs was implanted into a gap in the fascia of the femoral muscle in 5-week-old mice maintained on a Soy, Can, or H2-Soy diet. Newly formed bone volume, assessed by three-dimensional X-ray micro-computed tomography and three-dimensional reconstruction imaging for bone, was 4-fold greater in the Can and H2-Soy groups than in the Soy group. The plasma carboxylated osteocalcin (Gla-OC) and total OC (Gla-OC plus undercarboxylated osteocalcin [Glu-OC]) levels were significantly lower in the Can group than in the Soy group (p < 0.05). However, these levels did not significantly differ between the H2-Soy and Soy groups. The plasma Gla-OC/Glu-OC ratio in the Can and H2-Soy groups was significantly lower (in Can; p = 0.044) or was almost significantly lower (in H2-Soy; p = 0.053) than that in the Soy group. In conclusion, Can and H2-Soy accelerated BMP-induced bone formation in mice to a greater extent than Soy. Further research is required to evaluate whether the difference in accelerated ectopic bone formation is associated with altered levels of VK2 and VK-dependent protein(s) among the three dietary groups. |
| Databáze: | OpenAIRE |
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