Plant Bioactive Molecules Reduces Endoplasmic Reticulum Stress in Liver and Adipose Tissue of Obese Mice
| Autor: | Ivan Torre-Villalvazo, Berenice Perez, Claudia Tovar-Palacio, Nimbe Torres, Kenia Fuentes, Armando R. Tovar, Leonardo Rodriguez, Erik Alejandro Torre, Sandra Tobón |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Nutrition and Dietetics
Chemistry Endoplasmic reticulum Fatty liver Medicine (miscellaneous) Genistein Adipose tissue food and beverages Dietary Bioactive Components Tunicamycin Resveratrol medicine.disease Cell biology chemistry.chemical_compound Gene expression medicine Phosphorylation Food Science |
| Zdroj: | Curr Dev Nutr |
| Popis: | OBJECTIVES: To determine if diferent plant bioactive compounds can ammelirate endoplasmic reticulum stress markers in liver and adipose tissue of obese mice and mice administered with a low dose of tunicamicin. METHODS: C57BL6 mice were fed a control diet (7% fat) or a high-fat diet (21% fat) with and without genistein or resveratrol supplementation (0.1%) for 12 weeks. Pharmacologic ER stress was induced in mice fed the control diet by an ip injection of a low dose of tunicamycin and euthanized 8 or 24 h after tunicamycin administration. Adipose tissues and liver were harvested to determine the abundance of ER stress markers by western blot and real time PCR. RESULTS: Genistein and resveratrol reduced the abundance of phospho JNK and phospho PERK in liver and subcutaneous adipose tissue of obese mice and lean mice administered with tunicamycin. Both polyphenols increased the mRNA abundance of XBP1s and BiP and reduced that of CHOP in both organs. These changes in proten phosphorylation and gene expression were accompanied with reduced hepatic steatosis and adipocyte hypertrophy. CONCLUSIONS: The supplementation with plant polyphenols such as genistein or resveratrol reduced ER stress markers in liver and adipose tissue of obese mice and lean mice administerd with tunicamycin. FUNDING SOURCES: This work is supported by a grant from CONACYT, Mexico to ITV Grant No. A1-S-41,077. |
| Databáze: | OpenAIRE |
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