Bone Morphogenetic Protein-7 Reduces Cold Ischemic Injury in Rat Kidney
| Autor: | Josip Španjol, Dean Markić, Tanja Ćelić, Dragica Bobinac, Antun Gršković, Zeljko Fuckar, I. Prebilic |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Pathology Antioxidant Bone Morphogenetic Protein 7 medicine.medical_treatment Cold storage Kidney Bone morphogenetic protein Antioxidants Lipid peroxidation Superoxide dismutase chemistry.chemical_compound Internal medicine medicine Animals bone morphogenetic protein cold ischemia kidney Rats Wistar chemistry.chemical_classification Glutathione Peroxidase Transplantation biology Superoxide Dismutase business.industry Glutathione peroxidase Recombinant Proteins Rats Bone morphogenetic protein 7 Endocrinology medicine.anatomical_structure chemistry Reperfusion Injury biology.protein Surgery Lipid Peroxidation business |
| Zdroj: | Transplantation Proceedings. 43:2505-2509 |
| ISSN: | 0041-1345 |
| Popis: | Background Deceased donor kidneys have a high incidence of delayed graft function attributable to ischemia–reperfusion injury. Although preservation solution and cold storage reduce cold ischemic injury, the depletion of cellular energy and oxidative signalling leads to cellular damage. Because bone morphogenetic protein-7 has renoprotective effect, we have hypothesized that recombinant human bone morphogenetic protein-7 (rh BMP-7) will better preserve kidney tissue exposed to prolonged cold ischemia in comparison with University of Wisconsin (UW) solution. We evaluated how the duration of cold ischemia influences the cold ischemic injury. Methods Levels of lipid peroxidation, protein carbonyl content, as well as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were determined in the kidneys by spectrophotometry after 6, 12, and 24 hours of cold ischemia. Results Time-dependent increases in the levels of lipid peroxidation and protein carbonyl content at all time points were significantly lower in rhBMP-7–perfused kidneys versus the UW-treated group. SOD activity after 6 hours, as well as GSH-Px activity after 24 hours of cold ischemia was significantly higher in the kidney tissue perfused by rhBMP-7 than in UW group. Conclusion rhBMP-7 significantly decreases cellular damage in rat kidney versus UW preservation solution and this is attributed to lowering of cold ischemia injury and maintaining prolonged tissue antioxidant activity. |
| Databáze: | OpenAIRE |
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