Application of Paclitaxel-loaded EGFR Peptide-conjugated Magnetic Polymeric Liposomes for Liver Cancer Therapy
| Autor: | Zhenlv Lin, Jian Ding, Jie Xie, Xunru Huang, Shanshan He, Guo-Ping Sun, Xiaofei Liang, Dan Li |
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| Rok vydání: | 2020 |
| Předmět: |
Carcinoma
Hepatocellular Paclitaxel Polymers medicine.medical_treatment Polyethylene glycol Biochemistry Targeted therapy Mice chemistry.chemical_compound Nude mouse Cell Line Tumor PEG ratio Genetics medicine Animals Humans Particle Size Magnetite Nanoparticles Liposome biology Liver Neoplasms medicine.disease biology.organism_classification Antineoplastic Agents Phytogenic Xenograft Model Antitumor Assays ErbB Receptors Liver chemistry Liposomes Drug delivery Cancer research Peptides Liver cancer |
| Zdroj: | Current Medical Science. 40:145-154 |
| ISSN: | 2523-899X 2096-5230 |
| DOI: | 10.1007/s11596-020-2158-4 |
| Popis: | Developing the methodologies that allow for safe and effective delivery of therapeutic drugs to target sites is a very important research area in cancer therapy. In this study, polyethylene glycol (PEG)-coated magnetic polymeric liposome (MPL) nanoparticles (NPs) assembled from octadecyl quaternized carboxymethyl chitosan (OQC), PEGylated OQC, cholesterol, and magnetic NPs, and functionalized with epithelial growth factor receptor (EGFR) peptide, were successfully prepared for in-vivo liver targeting. The two-step liver targeting strategy, based on both magnetic force and EGFR peptide conjugation, was evaluated in a subcutaneous hepatocellular carcinoma model of nude mouse. The results showed that EGFR-conjugated MPLs not only accumulated in the liver by magnetic force, but could also diffuse into tumor cells as a result of EGFR targeting. In addition, paclitaxel (PTX) was incorporated into small EGFR-conjugated MPLs (102.0±0.7 nm), resulting in spherical particles with high drug encapsulation efficiency (>90%). The use of the magnetic targeting for enhancing the transport of PTX-loaded EGFR-conjugated MPLs to the tumor site was further confirmed by detecting PTX levels. In conclusion, PTX-loaded EGFR-conjugated MPLs could potentially be used as an effective drug delivery system for targeted liver cancer therapy. |
| Databáze: | OpenAIRE |
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