Evaluation of a modified direct agar proportion method for testing susceptibility of Mycobacterium tuberculosis from MGIT samples
| Autor: | Giueng Chueng Wang, Tzu Ting Chen, Mei Shiang Wang, Chia Ling Cheng, Han Lin Hsu, Yi Yuan Yang, Fang Lan Yu, Ming Chih Yu, Jau Ching Lee |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Veterinary medicine Time Factors food.ingredient Tuberculosis Cost-Benefit Analysis 030106 microbiology Antitubercular Agents Taiwan Microbial Sensitivity Tests Turnaround time Microbiology Mycobacterium tuberculosis 03 medical and health sciences food Immunology and Microbiology(all) medicine Cobas amplicor Humans Agar Immunology and Allergy Ethambutol General Immunology and Microbiology biology business.industry Isoniazid General Medicine Drug susceptibility biology.organism_classification medicine.disease Culture Media 030104 developmental biology Infectious Diseases business medicine.drug |
| Zdroj: | Journal of Microbiology, Immunology and Infection. 49(1):60-65 |
| ISSN: | 1684-1182 |
| DOI: | 10.1016/j.jmii.2014.01.003 |
| Popis: | Background/Purpose The emergence of resistance to anti-tuberculosis (TB) drugs has become an obstacle to effective TB control. Thus, there is an urgent need to identify patients and initiate adequate treatment for drug-resistant cases in a timely manner. The BACTEC MGIT 960 system is well known for its rapid culturing time, and is in widespread use in Taiwan. In this study, we evaluated the possibility of replacing the traditional indirect agar proportion method with a modified direct agar proportion method (MDAPM), as a technique for rapid testing the drug susceptibility of Mycobacterium tuberculosis without additional cost. Methods In this study, 432 positive MGIT 960 samples that were identified as M. tuberculosis complex using the MeDiPro M. tuberculosis Antigen Rapid Test or the Cobas Amplicor MTB test were evaluated. Each sample was tested separately by the MDAPM and indirect agar proportion method, between July 2008 and December 2008, to compare the consistency and total turnaround time. Results Four first-line anti-TB drugs—rifampin, isoniazid, ethambutol, and streptomycin—were tested. For the MDAPM and indirect agar proportion method, the respective consistencies for each drug were 99.31%, 98.38%, 98.38%, and 97.22%. Our results also indicated that the MDAPM leads to an average saving in working time of 2 weeks, compared with the traditional indirect agar proportion method. Conclusion In addition to having the potential to shorten turnaround time without compromising diagnostic quality, the MDAPM also provides a more efficient and cost-effective procedure. This modified procedure presents potential benefits for TB diagnosis in laboratories already equipped with the MGIT 960 system. |
| Databáze: | OpenAIRE |
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