Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish
| Autor: | Yuki Haga, Takashi Kondo, Nobuo Suzuki, Takeshi Nakano, Motoharu Suzuki, Atsuhiko Chiba, Vishwajit S. Chowdhury, Hiroyuki Mishima, Kazuichi Hayakawa, Kimi Kawabe, Chisato Matsumura, Akira Toriba, Masaki Nakano, Atsuhiko Hattori, Koji Yachiguchi, Toshio Sekiguchi, Noriko Matsumoto, Yoshiaki Tabuchi, Masahiro Tsurukawa, Shigehito Wada, T. Okuno, Masato Endo, Kei-ichiro Kitamura |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Bone metabolism Health Toxicology and Mutagenesis Osteoclasts Endogeny Bone resorption Bone remodeling chemistry.chemical_compound In vivo Fish scales Internal medicine Goldfish medicine Environmental Chemistry Animals PCB (118) Bone Resorption Osteoblasts Dimethyl sulfoxide organic chemicals Polychlorinated biphenyl General Medicine Metabolism Plasma calcium PCB mixtures in a complex world PCB (118) Plasma calcium Pollution Polychlorinated Biphenyls In vitro stomatognathic diseases Endocrinology chemistry Water Pollutants Chemical |
| Zdroj: | Environmental Science and Pollution Research International |
| ISSN: | 1614-7499 |
| Popis: | To analyze the effect of polychlorinated biphenyl (PCB) 118 on fish bone metabolism, we examined osteoclastic and osteoblastic activities, as well as plasma calcium levels, in the scales of PCB (118)-injected goldfish. In addition, effect of PCB (118) on osteoclasts and osteoblasts was investigated in vitro. Immature goldfish, in which the endogenous effects of sex steroids are negligible, were used. PCB (118) was solubilized in dimethyl sulfoxide at a concentration of 10 ppm. At 1 and 2 days after PCB (118) injection (100 ng/g body weight), both osteoclastic and osteoblastic activities, and plasma calcium levels were measured. In an in vitro study, then, both osteoclastic and osteoblastic activities as well as each marker mRNA expression were examined. At 2 days, scale osteoclastic activity in PCB (118)-injected goldfish increased significantly, while osteoblastic activity did not change significantly. Corresponding to osteoclastic activity, plasma calcium levels increased significantly at 2 days after PCB (118) administration. Osteoclastic activation also occurred in the marker enzyme activities and mRNA expressions in vitro. Thus, we conclude that PCB (118) disrupts bone metabolism in goldfish both in vivo and in vitro experiments. © 2012 The Author(s). |
| Databáze: | OpenAIRE |
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