Enhanced Vasculogenic Capacity Induced by 5-Fluorouracil Chemoresistance in a Gastric Cancer Cell Line

Autor: Fabio Cianchi, Francesco Coratti, Lucia Magnelli, Giampaolo Versienti, Elena Andreucci, Laura Papucci, Giuseppe Barbato, Sara Peri, Alessio Biagioni, Nicola Schiavone, Fabio Staderini, Lisa Giovannelli
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
medicine.medical_treatment
Metastasis
0302 clinical medicine
Biology (General)
Spectroscopy
vasculogenic mimicry
Neovascularization
Pathologic
chemoresistance
General Medicine
Thalidomide
Up-Regulation
Computer Science Applications
Chemistry
030220 oncology & carcinogenesis
Fluorouracil
medicine.drug
Paclitaxel
QH301-705.5
Antineoplastic Agents
Article
Catalysis
epithelial-to-endothelial transition
Inorganic Chemistry
03 medical and health sciences
Downregulation and upregulation
Stomach Neoplasms
Cell Line
Tumor
medicine
Humans
Vasculogenic mimicry
Physical and Theoretical Chemistry
Thymidine phosphorylase
Molecular Biology
QD1-999
Cisplatin
Thymidine Phosphorylase
Chemotherapy
business.industry
gastric cancer
Organic Chemistry
Endothelial Cells
Cancer
tumor angiogenesis
medicine.disease
030104 developmental biology
Drug Resistance
Neoplasm
Cancer cell
Cancer research
business
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 7698, p 7698 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 14
ISSN: 1661-6596
1422-0067
Popis: Chemotherapy is still widely used as a coadjutant in gastric cancer when surgery is not possible or in presence of metastasis. During tumor evolution, gatekeeper mutations provide a selective growth advantage to a subpopulation of cancer cells that become resistant to chemotherapy. When this phenomenon happens, patients experience tumor recurrence and treatment failure. Even if many chemoresistance mechanisms are known, such as expression of ATP-binding cassette (ABC) transporters, aldehyde dehydrogenase (ALDH1) activity and activation of peculiar intracellular signaling pathways, a common and universal marker for chemoresistant cancer cells has not been identified yet. In this study we subjected the gastric cancer cell line AGS to chronic exposure of 5-fluorouracil, cisplatin or paclitaxel, thus selecting cell subpopulations showing resistance to the different drugs. Such cells showed biological changes
among them, we observed that the acquired chemoresistance to 5-fluorouracil induced an endothelial-like phenotype and increased the capacity to form vessel-like structures. We identified the upregulation of thymidine phosphorylase (TYMP), which is one of the most commonly reported mutated genes leading to 5-fluorouracil resistance, as the cause of such enhanced vasculogenic ability.
Databáze: OpenAIRE
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