Association of ATG16L1 genetic variant with Helicobacter pylori infection in Egyptian patients with chronic gastritis disease
| Autor: | Ashraf Khalil, Aya Hamdy Abd-EL Aziz, Osama Elbahr, Gamal Youssef Abo Riaa, Heba Mohamed Abdallah, Samah Mohamed Awad |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
business.industry lcsh:R ATG16L1 lcsh:Medicine Chronic gastritis Rs2241880 Gene mutation medicine.disease ATG 03 medical and health sciences ATG16L1 SNP 030104 developmental biology Immune system Immunology Genotype Autophagy medicine H. Pylori Risk factor Allele business Genotyping |
| Zdroj: | Frontiers in Laboratory Medicine, Vol 2, Iss 4, Pp 134-140 (2018) |
| ISSN: | 2542-3649 |
| Popis: | Background Helicobacter pylori infection remains a major risk for gastroduodenal inflammation and ulceration with capability to disrupt the defensive autophagy pathway in the gastric mucosal cell thus producing resilient infection. A single-nucleotide mutation in the autophagy gene ATG16L1 which controls the host immune responses to viruses and bacteria was identified as a risk factor for increasing survival and persistence of many microbial infections. Aim To assess the association of ATG16L1 T300A gene mutation with the susceptibility to H. pylori infection in patients with chronic gastritis disease. Methods ATG16L1 T300A mutation was determined by TaqMan genotyping allele specific discrimination real-time PCR assay applying specific primers, (rs2241880 A/G) and probes for A and G allele. Result Patients with mutant homozygous GG genotype was associated with a 4.5 times increase in the risk of H. pylori infection relative to the wild homozygous AA and the heterozygous AG genotypes (p value = 0.009, OR = 4.472; 95% CI, 0.07–0.74). The carriage rate of the mutant G allele was significantly higher in H. pylori positive cases (64%) relative to (42%) in H. pylori negative cases (p value = 0.002, OR = 2.455, 95% CI, 0.3 (0.09–1.01), and this was associated with about a 2.5 times increase in the risk for H. pylori infection. Conclusion Genetic variant ATG16L1 T300A mutation increased the risk of H. pylori infection in Egyptian patients with chronic gastritis disease. |
| Databáze: | OpenAIRE |
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