Effect of Hypoxic Paracrine Media on Calcium-Regulatory Proteins in Infarcted Rat Myocardium
| Autor: | Byeong-Wook Song, Minji Seung, Moon Hyoung Lee, Hye Jin Hwang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Calmodulin Calcium homeostasis endoplasmic reticulum protein ATPase Paracrine Communication chemistry.chemical_element Stem cells Calcium Bioinformatics Calcium in biology Paracrine signalling Internal medicine Internal Medicine Paracrine communication Medicine biology business.industry Myocardial infarction Endocrinology chemistry biology.protein Original Article Stem cell Cardiology and Cardiovascular Medicine business Homeostasis |
| Zdroj: | Korean Circulation Journal |
| ISSN: | 1738-5555 1738-5520 |
| Popis: | BACKGROUND AND OBJECTIVES An increase in intracellular calcium concentration due to loss of Ca(2+) homeostasis triggers arrhythmia or cardiac cell death in the heart. Paracrine factors released from stem cells have beneficial cardioprotective effects. However, the mechanism of modulation of Ca(2+) homeostasis by paracrine factors in ischemic myocardium remains unclear. MATERIALS AND METHODS We isolated rat bone marrow-derived mesenchymal stem cells (MSCs), and prepared paracrine media (PM) from MSCs under hypoxic or normoxic conditions (hypoxic PM and normoxic PM). We induced rat myocardial infarction by left anterior descending ligation for 1 hour, and treated PM into the border region of infarcted myocardium (n=6/group) to identify the alteration in calcium-regulated proteins. We isolated and stained the heart tissue with specific calcium-related antibodies after 11 days. RESULTS The hypoxic PM treatment increased Ca(2+)-related proteins such as L-type Ca(2+) channel, sarcoplasmic reticulum Ca(2+) ATPase, Na(+)/K(+) ATPase, and calmodulin, whereas the normoxic PM treatment increased those proteins only slightly. The sodium-calcium exchanger was significantly reduced by hypoxic PM treatment, compared to moderate suppression by the normoxic PM treatment. CONCLUSION Our results suggest that hypoxic PM was significantly associated with the positive regulation of Ca(2+) homeostasis in infarcted myocardium. |
| Databáze: | OpenAIRE |
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