Detrimental Effects of 17β-Oestradiol after Permanent Middle Cerebral Artery Occlusion
| Autor: | I. Mhairi Macrae, Deborah Bingham, Hilary V O Carswell |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Pathology Ovariectomy Carbazoles Infarction Brain damage Placebo Central nervous system disease Amyloid beta-Protein Precursor medicine.artery Internal medicine medicine Animals Stroke Neurons Dose-Response Relationship Drug Estradiol business.industry Infarction Middle Cerebral Artery Anatomical pathology medicine.disease Immunohistochemistry Axons Rats Disease Models Animal Endocrinology Neurology Middle cerebral artery Female Neurology (clinical) medicine.symptom Cardiology and Cardiovascular Medicine business Perfusion |
| Zdroj: | Journal of Cerebral Blood Flow & Metabolism. 25:414-420 |
| ISSN: | 1559-7016 0271-678X |
| Popis: | Oestrogen is a complex hormone whose role as a neuroprotectant in experimental stroke has been published in numerous studies. However, although some clinical studies report a reduction in stroke incidence by oestrogen replacement therapy in postmenopausal women, others have found increased mortality and morbidity after stroke. Diathermy occlusion of the proximal middle cerebral artery (MCAO), one of the most reproducible rodent stroke models, has consequently been employed to investigate physiologic and supraphysiologic doses of 17β-oestradiol on ischaemic brain damage. Lister Hooded rats were ovariectomised (OVX) and a 21-day release pellet (placebo, 0.025 or 0.25 mg 17β-oestradiol) implanted in the neck. At 2 weeks after OVX, animals underwent MCAO and were perfusion fixed 24 hours later. Neuronal perikaryal damage was assessed from haematoxylin and eosin-stained sections and in adjacent sections, axonal pathology was assessed with amyloid precursor protein and Neurofilament 200 (NF-200) immunohistochemistry. 17β-Oestradiol induced a dose-dependent increase in neuronal perikaryal damage, 0.025 and 0.25 mg 17β-oestradiol increased damage by 20% ( P |
| Databáze: | OpenAIRE |
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