Monitoring ofBacillus thermodenitrificansOHT-1 in compost by whole cell hybridization
| Autor: | Kazuhiro Takamizawa, Junji Ohta, Masahiro Hatsu |
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| Rok vydání: | 2002 |
| Předmět: |
Hot Temperature
Immunology Colony Count Microbial Bacillus engineering.material DNA Ribosomal complex mixtures Applied Microbiology and Biotechnology Microbiology RNA Ribosomal 16S Genetics Molecular Biology Spores Bacterial Bacillaceae biology Compost Chemistry Thermophile fungi Nucleic Acid Hybridization General Medicine biology.organism_classification 16S ribosomal RNA Bacillales Aerobiosis Culture Media Refuse Disposal Biochemistry engineering Oligonucleotide Probes Oligomer restriction Fluorescein-5-isothiocyanate Bacteria |
| Zdroj: | Canadian Journal of Microbiology. 48:848-852 |
| ISSN: | 1480-3275 0008-4166 |
| Popis: | Thermophilic aerobic composting is a widely practiced method for the disposal of exhaust materials. We isolated a thermophilic bacteria strain from a compost sample under aerobic conditions at 60°C. On the basis of its 16S rRNA sequence and physiological characteristics, this strain was identified as Bacillus thermodenitrificans OHT-1. An 18-subunit oligonucleotide probe for 16S rRNA, labeled with fluorescein isothiocyanate, was developed for the detection of B. thermodenitrificans. Spores and vegetative cells of B. thermodenitrificans OHT-1 were detected in liquid culture and laboratory compost by whole cell hybridization using this oligonucleotide probe. The results obtained by whole cell hybridization were evaluated in growth experiments of B. thermodenitrificans OHT-1 in laboratory compost and were used to enumerate spores and vegetative cells.Key words: compost, Bacillus thermodenitrificans, 16S rRNA, whole cell hybridization. |
| Databáze: | OpenAIRE |
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