Immobilization of Stable Thylakoid Vesicles in Conductive Nanofibers by Electrospinning
| Autor: | G Douglas Winget, Srikoundinya Punnamaraju, Andrew J. Steckl, Nicholas M. Bedford |
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| Rok vydání: | 2011 |
| Předmět: |
Materials science
Light Polymers and Plastics Polymers Nanofibers Capsules Bioengineering macromolecular substances Thylakoids environment and public health Electron Transport Biomaterials PEDOT:PSS Spinacia oleracea Polymer chemistry Materials Chemistry Photosynthesis Electrochemical gradient Conductive polymer Vesicle Electric Conductivity food and beverages Cells Immobilized Electron transport chain Electrospinning Chemical engineering Thylakoid Nanofiber lipids (amino acids peptides and proteins) |
| Zdroj: | Biomacromolecules. 12:778-784 |
| ISSN: | 1526-4602 1525-7797 |
| Popis: | Electrospun fibers consisting of poly(3,4-ethylenedioxythiophene)/poly(styrene sulfonate) (PEDOT/PSS) and poly(ethylene oxide) (PEO) have been used to successfully encapsulate and stabilize thylakoid membrane vesicles isolated from spinach. Light-driven electronic properties were measured. Fibers with immobilized thylakoids show higher electrical conductivity compared with fibers without thylakoids under white light conditions. This is attributed to the electron-generating photosynthetic reactions from the thylakoids. Electron and optical microscopy show the presence of thylakoid vesicles within the fibers using lipid-specific stains. After electrospinning into fibers, the thylakoid vesicles still exhibit an ability to produce a light-driven electron gradient, indicating that activity is preserved during the electrospinning process. These electrospun fibers provide an excellent example of incorporating photosynthetic function into an artificial system. |
| Databáze: | OpenAIRE |
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