| Autor: |
Ming-Pan Choi, Man-Chun Chan, Timothy Ting-Leung Ng, Yuk-Yung Ng, Barry Kin-Chung Wong, Wing-Cheong Yam, Alex Yat-Man Ho, Kam-Tong Yip, Lam Kwong Lee, Wai-Shing Leung, Miranda Chong-Yee Yau, Sandy Ka Yee Chau, Eugene Yuk-Keung Tso, Tak-Lun Que, Gilman Kit Hang Siu, Kit-Man Sin, Kitty S. C. Fung, Shea Ping Yip, Hiu-Yin Lao, David Shum, Raymond W. Liu, Kenneth Siu Sing Leung, Alan K.L. Wu, Kwok Cheung Lung, Wing-Kin To, Kingsley King Gee Tam |
| Rok vydání: |
2020 |
| Předmět: |
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| Popis: |
Background: Initial cases of coronavirus disease 2019 (COVID-19) reported in Hong Kong were mostly imported cases from Mainland China. However, most cases reported in February 2020 were local infections with unknown source, indicating local community transmissions. This study aimed to report the clinical, epidemiological and phylogenomic characteristics of the local cases of COVID-19 in our community. Methods: We extracted the demographic, clinical and epidemiological data from 50 COVID-19 patients, who accounted for 53.8% of the cases in Hong Kong by the end of February 2020. We used both Nanopore and Illumina platforms to perform whole-genome sequencing (WGS) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from these patients. Phylogenetic relatedness among these local cases and their placement in the global phylogeny were examined. The evolutionary rate and divergence time of transmission were also determined. Findings: Of these 50 patients, only three (6.0%) had visited Wuhan while 43 (86.0%) did not have recent travel records. The average interval from symptom onset to hospital admission was 8.5 days. The most common signs and symptoms on admission were cough (74.0%) and fever (58.0%). Radiographic abnormality was found in 46 (92.0%) patients. Three (6.0%) patients required ICU admission. Phylogenetic analysis concurred with epidemiological investigation that 42 (84.0%) cases could be grouped into six transmission clusters. Forty-four (88.0%) cases harboured a common mutation Orf3a G251V. Global phylogeny of SARS-CoV-2 revealed that most (88.0%) cases in Hong Kong were clustered in two subclades with the strains from other countries. The estimated time to the most recent common ancestor (tMRCA) of COVID-2019 outbreak in Hong Kong was December 24, 2019 with an evolutionary rate of 3.04x10-3 substitutions per site per year. The reproduction number value was 1.84 as of February 28, 2020 in Hong Kong. Interpretation: We provided a territory-wide overview of COVID-19 in Hong Kong, which has borders connecting to Mainland China. Transmission in closed settings especially during family and religious gatherings is a hallmark of the recently reported cases. The reproduction number value indicated an ongoing outbreak in the community. Social distancing and vigilant epidemiological control are crucial to the containment of COVID-19 transmission |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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