Early and late HHV-6 gene transcripts in multiple sclerosis lesions and normal appearing white matter
| Autor: | Peter G. E. Kennedy, Margaret L. Opsahl |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology Genes Viral Transcription Genetic viruses Herpesvirus 6 Human Gene Expression law.invention law FISH = fluorescent in situ hybridization Polymerase chain reaction In Situ Hybridization Fluorescence Aged 80 and over MOBP = myelin-associated oligodendrocytic basic protein biology medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Articles Middle Aged Oligodendroglia medicine.anatomical_structure AMCA = 7-amino-4-methylcoumarin-3-acetic acid HHV-6 = human herpesvirus 6 RNA Viral Human herpesvirus 6 Female mRNA FISH Adult medicine.medical_specialty Multiple Sclerosis Roseolovirus Infections In situ hybridization GFAP = glial fibrillary acidic protein Immunofluorescence White matter HHV-6 IE = immediate early medicine Humans RNA Messenger Gene Southern blot Aged Cryopreservation Multiple sclerosis PCR = polymerase chain reaction medicine.disease biology.organism_classification FITC = fluorescein isothiocyanate NAWM = normal appearing white matter Neurology (clinical) oligodendrocyte |
| Zdroj: | Brain |
| ISSN: | 1460-2156 0006-8950 |
| Popis: | Multiple sclerosis is an inflammatory demyelinating disease of the CNS, the aetiology of which is believed to have both genetic and environmental components. We have investigated one of the candidate viruses for the environmental component of multiple sclerosis, the neurotropic human herpesvirus 6 (HHV-6). Utilizing fluorescent in situ hybridization (FISH) techniques, we have examined human post-mortem tissues for the presence of immediate early and late viral gene expression in multiple sclerosis patient normal appearing white matter (NAWM), lesional tissue and normal control brain samples. HHV-6 gene transcription was detected in all tissue samples and was restricted to oligodendrocytes, as determined by double mRNA FISH analysis. Quantitative analysis of viral mRNA expression indicated that both NAWM and lesional multiple sclerosis samples exhibited significantly higher levels of HHV-6 expression compared with the normal control samples. Lesional samples exhibited the highest levels of viral gene expression, with NAWM exhibiting an intermediate level between lesional and control tissues. Immunofluorescence against early and late HHV-6 proteins verified active translation of HHV-6 viral mRNA in oligodendrocytes. Southern blot analysis of nested polymerase chain reactions using extracted genomic DNA and cDNA confirmed the presence of the HHV-6 genome in all individuals, with the active expression profile mirroring the FISH results. The frequent high level of HHV-6 infection in multiple sclerosis samples suggests a possible role in pathogenesis. |
| Databáze: | OpenAIRE |
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