In vitro evaluation of Taxol combined with radiations in human squamous cell carcinoma spheroids
| Autor: | Jean-Louis Merlin, Christian Marchal, Genevieve Griffon-Etienne |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Radiation-Sensitizing Agents Paclitaxel Cell macromolecular substances Biology Radiation Tolerance Radiation sensitivity Spheroids Cellular medicine Tumor Cells Cultured Humans Radiosensitivity Clonogenic assay Spheroid Dose-Response Relationship Radiation Antineoplastic Agents Phytogenic In vitro Squamous carcinoma medicine.anatomical_structure Oncology Epidermoid carcinoma Head and Neck Neoplasms embryonic structures Cancer research Carcinoma Squamous Cell |
| Zdroj: | Cancer letters. 109(1-2) |
| ISSN: | 0304-3835 |
| Popis: | The effect of Taxol® on the radiation sensitivity of human squamous carcinoma of the head and neck region was determined in vitro, using clonogenic assays and multicellular tumor spheroids (MTS). Radiosensitivity parameters were determined by α and β for clonogenic assays, and by the residual/control volume ratios at 2 Gy (RSV 2 ) and the dose inducing 50% decrease in MTS number (SCD 50 ) for spheroids. In HTB43 and CAL27 colonies, the combination was antagonist. In spheroids, Taxol® induced a decrease of RSV 2 and SCD 50 in HTB43 and CAL27 MTS and their combinations with radiation were synergistic and additive, respectively. Therefore, the different results obtained by clonogenic assays and MTS may suggest higher drug incorporation through the multiple cell layers of the spheroids than in monolayers. |
| Databáze: | OpenAIRE |
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