A new method to process testicular sperm: combining enzymatic digestion, accumulation of spermatozoa, and stimulation of motility
| Autor: | Martina Wöber, Peter Oppelt, Eugen Plas, Heinz Strohmer, T. Ebner, Andreas Obruca, Sarah L. Steiner |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty Sperm Retrieval Pregnancy Rate Biology Cryopreservation Andrology Human fertilization Cell Movement Pregnancy Internal medicine medicine Humans Prospective Studies Sperm Injections Intracytoplasmic Blastocyst Birth Rate Infertility Male Sperm motility Azoospermia urogenital system Pregnancy Outcome Obstetrics and Gynecology General Medicine Middle Aged medicine.disease Spermatozoa Sperm Pregnancy rate Endocrinology medicine.anatomical_structure Sperm Motility Female |
| Zdroj: | Archives of Gynecology and Obstetrics. 291:689-694 |
| ISSN: | 1432-0711 0932-0067 |
| Popis: | In azoospermia processing of the TESE material often results in a sample of reduced purity. This prospective study was set up to clarify whether a combination of enzymatic digestion, density gradient centrifugation and stimulation of motility (where indicated) is a feasible option in TESE patients. A total of 63 samples (showing spermatozoa) were processed by the present tripartite processing method. The resulting sperm sample of high purity was directly used for ICSI and subsequent cryopreservation when quality of the accumulated sperm sample allowed for it (n = 39 cycles). Compared to the control group blastocyst formation rate in the present tripartite processing technique was significantly (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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