Examining Cholinergic Synaptic Signaling in the Thalamic Reticular Nucleus
Autor: | Rajan Dasgupta, Yan Gang Sun, Frederik Seibt, Michael Beierlein |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Mammals Thalamic reticular nucleus Chemistry Neurotransmission Synaptic Transmission General Biochemistry Genetics and Molecular Biology Cholinergic Neurons 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Receptor Cannabinoid CB1 Postsynaptic potential Thalamic Nuclei Synaptic plasticity medicine Cholinergic Animals Synaptic signaling Cholinergic neuron Reticular activating system Neuroscience Cannabinoid Receptor Antagonists 030217 neurology & neurosurgery |
Zdroj: | Cold Spring Harbor protocols. 2017(6) |
ISSN: | 1559-6095 |
Popis: | This protocol describes how to obtain monosynaptic cholinergic responses in neurons of the thalamic reticular nucleus (TRN) by making use of extracellular stimulation techniques. These methods are easy to implement and allow for the study of various forms of cholinergic synaptic plasticity and modulation. For many synapses throughout the mammalian brain, short-term plasticity is mediated by endocannabinoids released from postsynaptic neurons that activate presynaptic type I cannabinoid receptors (CB1Rs), resulting in the inhibition of presynaptic Ca2+ channels and a reduction of release probability. Neurons in the TRN are known to liberate endocannabinoids that can control transmitter release at GABAergic terminals. However, expression of CB1Rs on cholinergic terminals contacting the TRN has not been demonstrated. Here we outline strategies aimed to record stable postsynaptic responses and to quantify changes in cholinergic synaptic strength, using presynaptic modulation of acetylcholine (ACh) release by a CB1R agonist as an illustrative example. |
Databáze: | OpenAIRE |
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