Preparation of photocrosslinked fish elastin polypeptide/microfibrillated cellulose composite gels with elastic properties for biomaterial applications
| Autor: | Natsumi Suzuki, Toshiaki Shimasaki, Megumi Mori, Mitsuhiro Shibata, Shinya Yano, Yasuko Kaimoto, Eri Shiratsuchi, Masanari Noto, Michio Yamada, Masashi Kakimoto, Makoto Iisaka, Naozumi Teramoto |
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| Rok vydání: | 2014 |
| Předmět: |
Magnetic Resonance Spectroscopy
Composite number Pharmaceutical Science elastin Biocompatible Materials composite gel Methacrylate Article chemistry.chemical_compound Drug Discovery Polymer chemistry Ultimate tensile strength Spectroscopy Fourier Transform Infrared Animals Cellulose Pharmacology Toxicology and Pharmaceutics (miscellaneous) Elastic modulus lcsh:QH301-705.5 fish peptide biology Chemistry Tuna microfibrillated cellulose Biomaterial Photochemical Processes Elasticity Chemical engineering lcsh:Biology (General) Self-healing hydrogels biology.protein hydrogel photocrosslinking Peptides Elastin Gels |
| Zdroj: | Marine Drugs Marine Drugs, Vol 13, Iss 1, Pp 338-353 (2015) Volume 13 Issue 1 Pages 338-353 |
| ISSN: | 1660-3397 |
| Popis: | Photocrosslinked hydrogels reinforced by microfibrillated cellulose (MFC) were prepared from a methacrylate-functionalized fish elastin polypeptide and MFC dispersed in dimethylsulfoxide (DMSO). First, a water-soluble elastin peptide with a molecular weight of ca. 500 g/mol from the fish bulbus arteriosus was polymerized by N,N′-dicyclohexylcarbodiimide (DCC), a condensation reagent, and then modified with 2-isocyanatoethyl methacrylate (MOI) to yield a photocrosslinkable fish elastin polypeptide. The product was dissolved in DMSO and irradiated with UV light in the presence of a radical photoinitiator. We obtained hydrogels successfully by substitution of DMSO with water. The composite gel with MFC was prepared by UV irradiation of the photocrosslinkable elastin polypeptide mixed with dispersed MFC in DMSO, followed by substitution of DMSO with water. The tensile test of the composite gels revealed that the addition of MFC improved the tensile properties, and the shape of the stress–strain curve of the composite gel became more similar to the typical shape of an elastic material with an increase of MFC content. The rheology measurement showed that the elastic modulus of the composite gel increased with an increase of MFC content. The cell proliferation test on the composite gel showed no toxicity. |
| Databáze: | OpenAIRE |
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