Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts
| Autor: | Camila Rodrigues Eckert, Marcelo J. P. Ferreira, Edmar Silva Sobral, Wilhelm J. Baader, Oriana A. Fávero, Glalci Alves de Souza, Paulete Romoff, Thuany Alves Silva, Sandro de Oliveira |
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| Rok vydání: | 2014 |
| Předmět: |
ABTS
Chromatography biology DPPH Baccharis Trolox® percentage chemical and pharmacologic phenomena General Chemistry Ascorbic acid biology.organism_classification law.invention Luminol lcsh:Chemistry Rutin chemistry.chemical_compound lcsh:QD1-999 chemistry law luminol chemiluminescence Organic chemistry antioxidant assays Quercetin Chemiluminescence |
| Zdroj: | Química Nova v.37 n.3 2014 Química Nova Sociedade Brasileira de Química (SBQ) instacron:SBQ Química Nova, Volume: 37, Issue: 3, Pages: 497-503, Published: JUN 2014 Química Nova, Vol 37, Iss 3, Pp 497-503 (2014) |
| ISSN: | 0100-4042 |
| DOI: | 10.5935/0100-4042.20140076 |
| Popis: | The efficiency of the chemiluminescence luminol method and colorimetric DPPH and ABTS methods in evaluating the antiradical capacity of pure compounds and plant extracts with antioxidant potential is compared. In case of pure compounds, the values of parameter 'n' (number of radicals quenched per molecule of antiradical) for ascorbic acid, p-hydroquinone, catechol, quercetin, and rutin are similar when measured by colorimetric assays; however, considerably lower values of n are obtained with the luminol assay. The antiradical activity of extracts from male and female individuals of Baccharis burchelli and Baccharis crispa were determined by the luminol assay and expressed using the new Trolox® percentage (%Trolox®) parameter. |
| Databáze: | OpenAIRE |
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