Analysis of complete genome sequence and major surface antigens of Neorickettsia helminthoeca, causative agent of salmon poisoning disease
| Autor: | Naomi Sengamalay, Sandra Ott, Sean C. Daugherty, Zhihui Cheng, Claire M. Fraser, Sushma Nagaraj, Lisa Sadzewicz, Al Godinez, Katherine Bachman, Mingqun Lin, Luke J. Tallon, Yasuko Rikihisa, Julie C. Dunning Hotopp |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Neorickettsia Blotting Western Bioengineering Applied Microbiology and Biotechnology Biochemistry Neorickettsia helminthoeca Genome DNA sequencing Bacterial genetics 03 medical and health sciences Dogs medicine Animals Dog Diseases Research Articles Comparative genomics Genetics Whole genome sequencing Antigens Bacterial biology Whole Genome Sequencing biology.organism_classification medicine.disease Antibodies Bacterial 3. Good health 030104 developmental biology Anaplasmataceae Infections Antigens Surface Salmon poisoning disease Genome Bacterial Metabolic Networks and Pathways Biotechnology Research Article |
| Zdroj: | Microbial Biotechnology |
| ISSN: | 1751-7915 |
| Popis: | Summary Neorickettsia helminthoeca, a type species of the genus Neorickettsia, is an endosymbiont of digenetic trematodes of veterinary importance. Upon ingestion of salmonid fish parasitized with infected trematodes, canids develop salmon poisoning disease (SPD), an acute febrile illness that is particularly severe and often fatal in dogs without adequate treatment. We determined and analysed the complete genome sequence of N. helminthoeca: a single small circular chromosome of 884 232 bp encoding 774 potential proteins. N. helminthoeca is unable to synthesize lipopolysaccharides and most amino acids, but is capable of synthesizing vitamins, cofactors, nucleotides and bacterioferritin. N. helminthoeca is, however, distinct from majority of the family Anaplasmataceae to which it belongs, as it encodes nearly all enzymes required for peptidoglycan biosynthesis, suggesting its structural hardiness and inflammatory potential. Using sera from dogs that were experimentally infected by feeding with parasitized fish or naturally infected in southern California, Western blot analysis revealed that among five predicted N. helminthoeca outer membrane proteins, P51 and strain‐variable surface antigen were uniformly recognized. Our finding will help understanding pathogenesis, prevalence of N. helminthoeca infection among trematodes, canids and potentially other animals in nature to develop effective SPD diagnostic and preventive measures. Recent progresses in large‐scale genome sequencing have been uncovering broad distribution of Neorickettsia spp., the comparative genomics will facilitate understanding of biology and the natural history of these elusive environmental bacteria. |
| Databáze: | OpenAIRE |
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