A sandwich immunoassay for brucellosis diagnosis based on immune magnetic beads and quantum dots
| Autor: | Juan Wang, Li Li, Dandan Song, Kun Xu, Chao Zhao, Juan Li, Yushen Liu, Dehui Yin, Xiuling Song |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Clinical Biochemistry Staphylococcal protein Pharmaceutical Science Diagnostic Specificity Nanotechnology Sensitivity and Specificity 01 natural sciences Gastroenterology Brucellosis Analytical Chemistry 03 medical and health sciences Immune system Internal medicine Quantum Dots Drug Discovery medicine Humans Cutoff Sandwich immunoassay Spectroscopy Immunoassay Immunomagnetic Separation Chemistry 010401 analytical chemistry medicine.disease Antibodies Bacterial Brucella antibody 0104 chemical sciences Fluorescence intensity 030104 developmental biology |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 141:79-86 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2017.03.002 |
| Popis: | Brucellosis is a serious zoonosis with a rapid increase in incidence across epidemic regions. Currently, there are numerous methods for diagnosing brucellosis. However, these studies often have a few defects, such as low sensitivity, poor specificity, time consuming, laborious, and even potential biological risk. To overcome these shortcomings, we have developed a rapid, sensitive and accurate diagnosis procedure for brucellosis based on the immune magnetic beads (IMB) probe and quantum dots (QDs) – staphylococcal protein A (SPA) probe. With the presence of Brucella antibody in the tested serum, the QDs-SPA probe links to the IMB probe and an immune-sandwich complex is formed. As a result, the fluorescence intensity from QDs increased significantly and was correlated with the amount of Brucella antibody. Under the optimized conditions, 248 blood samples were detected and the diagnosis effect was evaluated. The area under the receiver-operating characteristic (ROC) curve was 0.970 (95% confidence interval (CI), 0.9479–0.9920). The diagnostic sensitivity was 96.15% (95% CI, 91.82–98.58%), the diagnostic specificity was 94.12% (95% CI, 87.64–97.81%) with a fluorescence intensity cutoff value of 150.4 and the detection time was only 100 min. This diagnostic procedure can be satisfactorily applied to the diagnosis of brucellosis. |
| Databáze: | OpenAIRE |
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