Diagnostic performance of patient self‐collected oral swab (tongue and cheek) in comparison with healthcare worker‐collected nasopharyngeal swab for severe acute respiratory syndrome coronavirus‐2 detection

Autor: Arati, Mane, Shilpa, Jain, Ankita, Jain, Vijay, Nema, Swarali, Kurle, Vandana, Saxena, Michael, Pereira, Atul, Sirsat, Gaurav, Pathak, Vikalp, Bhoi, Shailaja, Bhavsar, Samiran, Panda
Rok vydání: 2022
Předmět:
Zdroj: APMIS. 130:671-677
ISSN: 1600-0463
0903-4641
Popis: The present study was conducted to compare the performance of patient self-collected oral swab (OS) with healthcare worker (HCW)-collected nasopharyngeal swab (NPS) for SARS-CoV-2 detection by reverse transcription polymerase chain reaction (RT-PCR) in real-world setting. Paired OS and NPS were collected from 485 consecutive individuals presenting with symptoms of coronavirus disease-19 (COVID-19) or asymptomatic contacts of COVID-19 cases. Both specimens were processed for RT-PCR and cycle threshold (Ct) value for each test was obtained. Positive percent agreement (PPA), negative percent agreement (NPA), overall percent agreement (OPA) and kappa were calculated for OS RT-PCR compared with NPS RT-PCR as reference. A total of 116/485 (23.9%) participants were positive by NPS RT-PCR. OS had PPA of 71.6%, NPA of 98.8%, OPA of 92.4% and kappa of 0.771. Almost all participants (483/485, 99.6%) reported OS as a convenient and comfortable sample for SARS-CoV-2 testing over NPS. All participants with Ct values25 and majority (90.8%) with Ct values30 were detected by OS. To conclude, OS self-sampling was preferred in comparison with NPS due the ease and comfort during collection. The performance of OS RT-PCR for SARS-CoV-2 detection, however, was sub-optimal in comparison with NPS RT-PCR.
Databáze: OpenAIRE
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