Primary culture and cryopreservation of mouse astrocytes under serumfree conditions
| Autor: | Masao Takeuchi, Touho Yoshida |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Cell Survival
Clinical Biochemistry Biomedical Engineering Fluorescent Antibody Technique Bioengineering Biology Fibroblast growth factor Cryopreservation Mice Epidermal growth factor Cell Adhesion medicine Animals Dimethyl Sulfoxide Cells Cultured chemistry.chemical_classification Mice Inbred C3H Glial fibrillary acidic protein Brain Cell Biology Molecular biology Culture Media Chemically defined medium Blood medicine.anatomical_structure chemistry Cell culture Transferrin Astrocytes biology.protein Cell Division Biotechnology Astrocyte |
| Zdroj: | Cytotechnology. 5:99-106 |
| ISSN: | 1573-0778 0920-9069 |
| Popis: | The methods of primary culture and cryopreservation of mouse astrocytes under serum-free conditions were examined. Cerebra from newborn C3H/He mice were employed as the source of astrocytes. The cultured cells were able to grow in a serum-free, chemically defined medium containing transferrin, hydrocortisone, biotin, sodium selenite, insulin, fibroblast growth factor and epidermal growth factor. After the culture was maintained in the medium for 3 weeks, purity was assessed using immunofluorescence staining. The great majority of the cells (greater than 98%) contained glial fibrillary acidic protein and S-100 protein which are cell markers of astrocytes. To cryopreserve the enriched astrocytes under serum-free conditions, various cryoprotectants were examined. The combination of 10% dimethylsulfoxide and 0.1% methylcellulose gave the highest survival rate. These methods of primary culture and cryopreservation will be useful in physiological and biochemical studies which require mouse astrocytes. |
| Databáze: | OpenAIRE |
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