Stevenleaf from Gynostemma Pentaphyllum inhibits human hepatoma cell (HepG2) through cell cycle arrest and apoptotic induction
| Autor: | Fan Zhang, Yuan-Yuan Zhang, Mahrukh Naudhani, Kiran Thakur, Carlos L. Cespedes-Acuña, Sayed Sajid Hussain, Zhao-Jun Wei |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cell cycle checkpoint
Cyclin E 030309 nutrition & dietetics Cyclin A HepG2 cell Bcl-xL Apoptosis lcsh:TX341-641 Cell cycle 03 medical and health sciences 0404 agricultural biotechnology Cyclin D1 FADD Stevenleaf 0303 health sciences Cyclin-dependent kinase 1 biology Chemistry 04 agricultural and veterinary sciences 040401 food science Cell biology Gynostemma Pentaphyllum biology.protein lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Food Science and Human Wellness, Vol 9, Iss 3, Pp 295-303 (2020) |
| ISSN: | 2213-4530 |
| Popis: | The anticancer activity of stevenleaf (SV) on the basis of cell viability, cell cycle, and apoptosis induction in HepG2 cancer cells were evaluated. SV controlled the growth of HepG2 cells with IC50 of 139.82 μmol/L for 24 h, IC50 of 119.12 μmol/L for 48 h and cell cycle arrested at G0/G1 phase, induced cell apoptosis and enhanced intracellular ROS generation. For cell cycle arrest, the mRNA expression levels of p21, p27 and p53 were up-regulated, while the expression levels of Cyclin A, Cyclin D1, Cyclin E and CDK1/2 were down-regulated. SV efficiently up-regulated TNF R1, TRADD1 and FADD and down-regulated Caspase8 for cell death receptors; similarly, up-regulated Bax, Bak, Cyt c, Apaf1, Caspase3 and Caspase9, and down-regulated Bcl2, Bcl xl and Bad for mitochondrial signal pathway. SV induced the mTOR-mediated cell apoptosis in HepG2 cells via activation of Akt and AMPK. The mechanistic explanation for the anticancer activity of SV as functional food can be derived from above results. |
| Databáze: | OpenAIRE |
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