The mitochondrial ATP / ADP carrier: Interaction with detergents and purification by a novel procedure
| Autor: | Theo Wallimann, Manuel Rojo |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Detergents
Biophysics Mitochondria Liver Cell Fractionation Biochemistry Isozyme Mitochondria Heart Antigen Western blot medicine Animals heterocyclic compounds Antiserum Chromatography medicine.diagnostic_test Chemistry Liver and kidney Cell Biology biochemical phenomena metabolism and nutrition bacterial infections and mycoses Mitochondria Mitochondria Muscle Rats Blue-Sepharose carbohydrates (lipids) Solubilization bacteria Cattle ATP–ADP translocase Chickens Mitochondrial ADP ATP Translocases |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1187:360-367 |
| ISSN: | 0005-2728 |
| DOI: | 10.1016/0005-2728(94)90010-8 |
| Popis: | The interaction of several classes of detergents with mitochondrial ATP / ADP carrier (AAC) was studied. The detergents that were best suited for solubilization of active AAC differed in several physico-chemical properties, but contained relatively rigid or planar hydrophobic (sub)moieties. Based on specific binding of AAC to Blue Sepharose, a novel method for the purification of the AAC was developed. The new method gave AAC samples which were devoid of non-essential lipids and allowed to purify AAC isoenzymes from several species and tissues to a significantly higher degree of purity than that achieved up to now. Western blot analysis of purified AACs with an antiserum against chicken heart AAC confirmed that immunological variability is more important between tissues than between species. In contrast to liver and kidney AACs, brain AAC displayed similar antigenic properties to heart AAC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|