Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study
| Autor: | Ștefan-Ioan Stratul, Florina Bojin, Darian Rusu, Nela-Pusa Gaje, Marius Boariu, Ștefan Milicescu, Bogdan Calenic, Virgil Paunescu, Costin Caruntu, Petra Surlin, Horia Calniceanu, Alexandra Roman, Andreea Cristiana Didilescu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Cancer Research
T cell 0206 medical engineering Population wound healing 02 engineering and technology Peripheral blood mononuclear cell chemistry.chemical_compound Immune system Immunology and Microbiology (miscellaneous) medicine Propidium iodide Viability assay education T-lymphocytes education.field_of_study oral mucosa apoptosis General Medicine Articles collagen matrix 021001 nanoscience & nanotechnology 020601 biomedical engineering Molecular biology medicine.anatomical_structure chemistry 0210 nano-technology Wound healing CD8 |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | Previous experimental models showed that activation of the immune system, particularly T cells, is required for optimal healing following wounds or surgery in the oral cavity. Therefore, studies to explore the interactions between the immune system and the collagen matrix are mandated. The specific aim of the present study was to analyze the interactions between T lymphocytes and a resorbable three-dimensional (3D) collagen matrix routinely used for soft tissue regeneration during periodontal surgery. Peripheral venous blood samples were collected from five patients. Following Ficoll-Paque separation, mononuclear cells were grown on fully resorbable 3D collagen matrices for 5 days. Lymphocytes were analyzed by flow cytometry for different surface markers, including CD4, CD8, CD38 and CD69. Cell viability and late apoptosis/necrosis were assessed in each group using an apoptosis assay based on Annexin V/propidium iodide staining. After 5 days in contact with the collagen matrix, the T cells expressed different surface markers. The overall T cell population increased significantly in the collagen matrix group compared to the respective controls (31.9±6.5 vs. 38.7±3.8%). CD8 and CD69 also increased significantly compared to their controls (CD69: 19.7±3.0 vs. 27.1±4.5% for collagen vs. control groups). At the same time, CD4 and CD38 expression was similar in both groups. Viability and apoptosis/necrosis were also identical in the samples and controls. These results show that the interaction between the collagen matrix and the immune cells stimulated activation of T cells and did not impair the healing process. |
| Databáze: | OpenAIRE |
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