Detection of flavokavins (A, B, C) in cultivars of kava (Piper methysticum) using high performance thin layer chromatography (HPTLC)

Autor: T.K.T. Do, Laurent Legendre, Vincent Lebot
Přispěvatelé: Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS )
Jazyk: angličtina
Rok vydání: 2014
Předmět:
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Identification
[ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology
[ SDV.TOX ] Life Sciences [q-bio]/Toxicology
Composition des aliments
Plant Roots
01 natural sciences
Analytical Chemistry
HPTLC
Chalcone
0302 clinical medicine
Hépatotoxine
High performance thin layer chromatography
Cultivar
Contrôle de qualité
Routine analysis
[ SDV.SA ] Life Sciences [q-bio]/Agricultural sciences
Kava
[SDV.EE]Life Sciences [q-bio]/Ecology
environment
Traditional medicine
Composition chimique
General Medicine
Lactone
Quality
Flavokavins
030220 oncology & carcinogenesis
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Q03 - Contamination et toxicologie alimentaires
Qualité
Quality Control
Flavokavin B
F60 - Physiologie et biochimie végétale
Piper methysticum
[SDV.BID]Life Sciences [q-bio]/Biodiversity
Biology
Pacific Islands
[ SDV.EE ] Life Sciences [q-bio]/Ecology
environment
Beverages
03 medical and health sciences
Kavalactones
Toxicité
Botany
Food Quality
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Q04 - Composition des produits alimentaires
[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry
Molecular Biology
[ SDV.BID ] Life Sciences [q-bio]/Biodiversity
Flavonoids
Chromatographie en couche mince
010401 analytical chemistry
Flavonoïde
0104 chemical sciences
Collection de matériel génétique
Chromatography
Thin Layer
U30 - Méthodes de recherche
Food Science
Zdroj: Food Chemistry
Food Chemistry, Elsevier, 2014, 151, pp.554-560. ⟨10.1016/j.foodchem.2013.11.120⟩
Food Chemistry, Elsevier, 2014, 151, pp.554-560. 〈10.1016/j.foodchem.2013.11.120〉
ISSN: 0308-8146
Popis: International audience; Kava (Piper methysticum) is used to prepare the traditional beverage of the Pacific islands. In Europe, kava has been suspected to cause hepatoxicity with flavokavin B (FKB) considered as a possible factor. The present study describes an HPTLC protocol for rapid screening of samples. The objectives are: to detect the presence of flavokavins in extracts and to compare the FKB levels in different cultivars. Overall, 172 samples originating from four cultivars groups (noble, medicinal, two-days and wichmannii), were analysed. Results indicate that the ratio FKB/kavalactones is much higher in two-days (0.39) and wichmannii (0.32) compared to nobles (0.09) and medicinal cultivars (0.10). For each group, the ratios flavokavins/kavalactones do not change significantly between roots, stumps or basal stems and among clones, indicating that they are genetically controlled. This protocol has good accuracy and is cost efficient for routine analysis. We discuss how it could be used for quality control.
Databáze: OpenAIRE
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