Co-Compartmentation of Terpene Biosynthesis and Storage via Synthetic Droplet
| Autor: | Man Li, Yining Zeng, Shi You Ding, Joshua S. Yuan, Susie Y. Dai, Cheng Hu, Cheng Zhao, Yongkyoung Kim, Xin Wang, Connor Gorman |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Squalene Biomedical Engineering Bioengineering Biochemistry Genetics and Molecular Biology (miscellaneous) Terpene 03 medical and health sciences chemistry.chemical_compound Biosynthesis Tobacco Fluorescence microscope chemistry.chemical_classification Chemistry Terpenes Geranyltranstransferase General Medicine Plants Genetically Modified Biosynthetic Pathways Cell Compartmentation Chloroplast 030104 developmental biology Enzyme Yield (chemistry) Biophysics Oleosin Mesophyll Cells Subcellular Fractions |
| Zdroj: | ACS synthetic biology. 7(3) |
| ISSN: | 2161-5063 |
| Popis: | Traditional bioproduct engineering focuses on pathway optimization, yet is often complicated by product inhibition, downstream consumption, and the toxicity of certain products. Here, we present the co-compartmentation of biosynthesis and storage via a synthetic droplet as an effective new strategy to improve the bioproduct yield, with squalene as a model compound. A hydrophobic protein was designed and introduced into the tobacco chloroplast to generate a synthetic droplet for terpene storage. Simultaneously, squalene biosynthesis enzymes were introduced to chloroplasts together with the droplet-forming protein to co-compartmentalize the biosynthesis and storage of squalene. The strategy has enabled a record yield of squalene at 2.6 mg/g fresh weight without compromising plant growth. Confocal fluorescent microscopy imaging, stimulated Raman scattering microscopy, and droplet composition analysis confirmed the formation of synthetic storage droplet in chloroplast. The co-compartmentation of synthetic storage droplet with a targeted metabolic pathway engineering represents a new strategy for enhancing bioproduct yield. |
| Databáze: | OpenAIRE |
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