Multiple electrode aggregometry: a new device to measure platelet aggregation in whole blood
| Autor: | Hajna Losonczy, Wolfgang Siess, Orsolya Tóth, Andreas Calatzis, Sandra M Penz |
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| Rok vydání: | 2006 |
| Předmět: |
Time Factors
Platelet Aggregation Platelet Function Tests Hirudin Pharmacology In Vitro Techniques Sodium Citrate Sensitivity and Specificity chemistry.chemical_compound Reference Values Sodium citrate medicine Electric Impedance Humans Platelet Spontaneous platelet aggregation Citrates Electrodes Whole blood Aspirin Dose-Response Relationship Drug Chemistry Apyrase Platelet Count food and beverages Anticoagulants Reproducibility of Results Hematology Hirudins Peptide Fragments Adenosine Diphosphate Blood Preservation Hemostasis Immunology Collagen Platelet Aggregation Inhibitors medicine.drug |
| Zdroj: | Thrombosis and haemostasis. 96(6) |
| ISSN: | 0340-6245 |
| Popis: | SummarySeveral methods are used to analyse platelet function in whole blood. A new device to measure whole blood platelet aggregation has been developed, called multiple electrode platelet aggregometry (MEA). Our aim was to evaluate MEA in comparison with the single platelet counting (SPC) method for the measurement of platelet aggregation and platelet inhibition by aspirin or apyrase in diluted whole blood. Platelet aggregation induced by different concentrations of ADP, collagen and TRAP-6 and platelet inhibition by apyrase or aspirin were determined in citrateor hirudin-anticoagulated blood by MEA and SPC. MEA indicated that spontaneous platelet aggregation was lower, and stimulated platelet aggregation was higher in hirudin- than citrate-anticoagulated blood. In hirudin-anticoagulated, but not citrate-anticoagulated blood, spontaneous platelet aggregation measured by MEA was inhibited by apyrase. For MEA compared with SPC the dose response-curves of agonist-induced platelet aggregation in citrate- and hirudin-blood showed similar EC50 values for TRAP, and higher EC50 values for ADP (non-significant) and collagen (p |
| Databáze: | OpenAIRE |
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