A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization
| Autor: | João Alfredo Moraes, Luís Eduardo Ribeiro da Cunha, Marcos Monteiro-Machado, Rui Seabra Ferreira, Paulo A. Melo, Benedito Barraviera, Matheus Da Silva Tavares-Henriques, Jhonatha Da Mota Teixeira-Cruz, Luis Eduardo M. Quintas, Marcelo A. Strauch |
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| Přispěvatelé: | Federal University of Rio de Janeiro, Scientific Board, Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Swine Health Toxicology and Mutagenesis Antivenom Drug Evaluation Preclinical Poison control lcsh:Medicine Venom Pharmacology Toxicology chemistry.chemical_compound Mice Medicine Bites and Stings Cytotoxicity 0303 health sciences Antivenins honeybee venom 030302 biochemistry & molecular biology Bees Bee Venoms Phospholipases Models Animal Female Apis mellifera Brazil ApilicApilic antivenom Injections Intradermal Cell Survival Myotoxin Hyaluronoglucosaminidase Hemolysis complex mixtures Melittin Antibodies Article Cell Line Lethal Dose 50 03 medical and health sciences Immunoglobulin Fab Fragments In vivo Neutralization Tests preclinical study Animals Horses Envenomation 030304 developmental biology Dose-Response Relationship Drug envenomation business.industry Apilic antivenom lcsh:R Melitten chemistry melittin LLC-PK1 Cells business |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Toxins, Vol 13, Iss 30, p 30 (2021) Toxins Volume 13 Issue 1 |
| Popis: | Massive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new ApilicApilic antivenom, which is composed of F(ab&rsquo )2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25&ndash 0.5 &mu L of intravenous ApilicApilic antivenom/&mu g honeybee venom. In vivo injection of 0.1&ndash 1 &mu g/g bee venom induced myotoxicity, hemoconcentration, paw edema, and increase of vascular permeability which were antagonized by ApilicApilic antivenom. Cytotoxicity, assessed in renal LLC-PK1 cells and challenged with 10 &mu g/mL honeybee venom or melittin, was neutralized by preincubation with ApilicApilic antivenom, as well the hemolytic activity. Apilic antivenom inhibited phospholipase and hyaluronidase enzymatic activities. In flow cytometry experiments, ApilicApilic antivenom neutralized reduction of cell viability due to necrosis by honeybee venom or melittin. These results showed that this antivenom is effective inhibitor of honeybee venom actions. Thus, this next generation of ApilicApilic antivenom emerges as a new promising immunobiological product for the treatment of massive, Africanized honeybee attacks. |
| Databáze: | OpenAIRE |
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