Restored invasion of mouse MO4 cells into chick heart in vitro through mutual conditioning at reduced temperature
| Autor: | Marc Mareel, Marc De Mets, J G Bolscher, Lou A. Smets, Erik Bruyneel |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Cancer Research
medicine.medical_specialty Chick Embryo Biology Organ culture Models Biological Fucose Cell Line chemistry.chemical_compound Mice Organ Culture Techniques Cell Movement Internal medicine medicine Animals Neoplasm Invasiveness Incubation Glycoproteins Cell invasion chemistry.chemical_classification Hematology Myocardium Temperature Heart General Medicine In vitro Cell biology Kinetics Oncology chemistry Immunology Conditioning Sarcoma Experimental Glycoprotein Cell Division |
| Zdroj: | Clinicalexperimental metastasis. 7(3) |
| ISSN: | 0262-0898 |
| Popis: | Invasion of malignant mouse MO4 cells into embryonic chick heart fragments in confronting organ cultures was arrested for 7 days when the temperature of incubation was lowered to 28 degrees C. Afterwards invasion resumed and progression between days 10 and 17 at 28 degrees C was comparable to that between days 0 and 7 at 37 degrees C. This pattern of progression of MO4 cell invasion at 28 degrees C was unaltered when either MO4 cells or heart fragments or both were preincubated separately at 28 degrees C for 14 days before confrontation with each other. Invasion at 28 degrees C resumed only when MO4 cells and heart tissue had been in immediate contact for at least 7 days. Metabolic labelling with [3H]fucose showed a correlation in time between transient suppression of invasion and transient inhibition of incorporation of fucosylation-precursor molecules into glycoproteins by MO4 cells. The latter activity was far less temperature-sensitive in heart cells. Our observations suggest that metabolic cooperation between invading MO4 cells and heart tissue is essential for progression of invasion in vitro. |
| Databáze: | OpenAIRE |
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