Nucleotide sequence of the gene, protein purification and characterization of the pSC101-encoded tetracycline resistance-gene-repressor
| Autor: | Wolfgang Hillen, Bernhard Unger, Jörn Becker |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
DNA
Bacterial R Factors Repressor Biology chemistry.chemical_compound stomatognathic system Protein purification polycyclic compounds Genetics Escherichia coli TetR Amino Acid Sequence Promoter Regions Genetic Gene Peptide sequence Base Sequence Nucleic acid sequence Protein primary structure General Medicine biochemical phenomena metabolism and nutrition Tetracycline Molecular biology Bacteriophage lambda Repressor Proteins chemistry Gene Expression Regulation Genes Genes Bacterial DNA Transcription Factors |
| Zdroj: | Gene. 31(1-3) |
| ISSN: | 0378-1119 |
| Popis: | The nucleotide sequence of the pSC101-encoded tetracycline repressor gene ( tet R) was confirmed. The deduced amino acid sequence is compared to that of other repressor proteins. To overproduce the represser protein, tetR was placed under the control of bacteriophage λ promoter p L . tet repressor protein was purified to homogeneity and shown to bind specifically to two tet operators and also to tet racycline (Tc). The inducer function of Tc is demonstrated by the loss of the specific binding between the tet operator DNA and the tet repressor-Tc complex. |
| Databáze: | OpenAIRE |
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