Disruption of thymopoiesis in ST6Gal I-deficient mice
| Autor: | Eun Soo Han, Rebecca Naukam, C. jJstin Van De Wiele, Kenton S. Miller, T. Kent Teague, Julie H. Marino, Ashlee Taylor, Morgan Hickey, Brenda K. Davis, Chibing Tan |
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| Rok vydání: | 2008 |
| Předmět: |
Glycosylation
Sialyltransferase T cell Cellular differentiation Oligosaccharides Cell Count Thymus Gland Biochemistry Mice chemistry.chemical_compound medicine Animals Avidity beta-D-Galactoside alpha 2-6-Sialyltransferase Oligonucleotide Array Sequence Analysis Mice Knockout biology Gene Expression Profiling T-cell receptor Cell Differentiation Original Articles Sialyltransferases Sialic acid Cell biology Mice Inbred C57BL Thymocyte medicine.anatomical_structure chemistry Immunology biology.protein Female |
| Zdroj: | Glycobiology. 18:719-726 |
| ISSN: | 1460-2423 0959-6658 |
| Popis: | Thymocyte development is accompanied by sequential changes in cell surface glycosylation. For example, medullary thymocytes have increased levels of alpha2,3-linked sialic acid and a loss of asialo core 1 O-glycans as compared to cortical thymocytes. Some of these changes have been linked to fine tuning of the T cell receptor avidity. We analyzed ST6Gal I transcript abundance and levels of alpha2,6-linked sialic acid across thymocyte subsets. We found that ST6Gal I transcript levels increased following T cell receptor beta-selection suggesting that this sialyltransferase may influence the development of early thymocyte populations. Indeed, low levels of alpha2,6-linked sialic acid were found in the earliest T lineage cells, and then increased in T cell receptor beta-selected cells. To determine whether ST6Gal I influences T cell development, we analyzed ST6Gal I-deficient mice for disruptions in thymocyte populations. We found reduced thymic cellularity in the ST6Gal I-deficient mice starting in the early thymocyte compartments. |
| Databáze: | OpenAIRE |
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