Aspirin and indomethacin reduce lung inflammation of mice exposed to cigarette smoke
| Autor: | Daniele C. Rezende, Vera Lúcia Gonçalves Koatz, Larissa Cardilo dos Reis, Agessandro Abrahao, Samuel Santos Valença, Luís Cristóvão Porto, Ingred G. Riça, Moisés C.M. Cavalcante, Luis Eduardo M. Quintas, Paulo Castro, Helena Nasser |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_treatment Indomethacin Inflammation Pharmacology Biochemistry Lipid peroxidation Mice chemistry.chemical_compound Indometacin Smoke Tobacco medicine Animals Aspirin Dose-Response Relationship Drug medicine.diagnostic_test Pneumonia Neutrophilia Mice Inbred C57BL Oxidative Stress Dose–response relationship Bronchoalveolar lavage Neutrophil Infiltration chemistry Immunology medicine.symptom Bronchoalveolar Lavage Fluid Prostaglandin E medicine.drug |
| Zdroj: | Biochemical Pharmacology. 77:1029-1039 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/j.bcp.2008.12.012 |
| Popis: | Neutrophil accumulation response to cigarette smoke (CS) in humans and animal models is believed to play an important role in pathogenesis of many tobacco-related lung diseases. Here we evaluated the lung anti-inflammatory effect of aspirin and indomethacin in mice exposed to CS. C57BL/6 mice were exposed to four cigarettes per day during 4 days and were treated i.p. with aspirin or indomethacin, administered each day 1h before CS exposure. Twenty four hours after the last exposure, cells and inflammatory mediators were assessed in bronchoalveolar lavage (BAL) fluid and the lungs used for evaluation of lipid peroxidation, p38 mitogen-activated protein kinase (MAPK) phosphorylation and nuclear transcription factor kappaB (NF-kappaB) activation. Exposure to CS resulted in a marked lung neutrophilia. Moreover, the levels of oxidative stress-related lipid peroxidation, prostaglandin E(2) (PGE(2)), interleukin 1beta (IL-1beta), monocyte chemotactic protein 1 (MCP-1), and activated NF-kappaB and p38 MAPK were greatly increased in CS group. Aspirin or indomethacin treatment led to a significant reduction of neutrophil influx, but only aspirin resulted in dramatic decrease of inflammatory mediators. Moreover, both drugs reduced lung p38 MAPK and NF-kappaB activation induced by CS. These results demonstrate that short-term CS exposure has profound airway inflammatory effects counteracted by the anti-inflammatory agents aspirin and indomethacin, probably through COX-dependent and -independent mechanisms. |
| Databáze: | OpenAIRE |
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