Cloning and nucleotide sequence of a linear DNA plasmid fromXanthophyllomyces dendrorhous (Phaffia rhodozyma)
| Autor: | M. R. Kula, L. M. Ducrey Santopietro |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Genetics
Cloning Base Sequence Basidiomycota Molecular Sequence Data Restriction Mapping Nucleic acid sequence Sequence Analysis DNA General Medicine Biology Molecular cloning Microbiology Molecular biology Open Reading Frames Restriction enzyme Open reading frame chemistry.chemical_compound Plasmid chemistry Extrachromosomal DNA Amino Acid Sequence Cloning Molecular DNA Fungal DNA Plasmids |
| Zdroj: | Folia Microbiologica. 46:277-288 |
| ISSN: | 1874-9356 0015-5632 |
| DOI: | 10.1007/bf02815614 |
| Popis: | Extrachromosomal elements were found in a strain of X. dendrorhous, and were characterized as linear DNA forming two well defined groups, pPh1 with 3 high-copy-number molecules, pPh11 (6.9 kb), pPh12 (5.7), pPh13 (4.7), and pPh2 with 2 low-copy-number molecules, pPh21 (3.6 kb), pPh22 (3.0). A 4077 bp fragment from pPh13 was cloned in pUC18 (pDK1) and sequenced (accession no. AJ 278,424). Seven putative ORF and some possible regulator sequences were defined. |
| Databáze: | OpenAIRE |
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