Stabilization of methionine-enkephalin in human and rat blood
| Autor: | Shaker A. Mousa, Glen R. Van Loon, Vincent J. Aloyo |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Male
Enkephalin Methionine Biology Peptide hormone Citric Acid General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Aprotinin medicine Animals Humans Citrates General Pharmacology Toxicology and Pharmaceutics Blood Specimen Collection Human blood Temperature Rats Inbred Strains Enkephalins General Medicine Methionine enkephalin Rat blood Rats chemistry Biochemistry Chromatography Gel Chromatography Thin Layer Citric acid medicine.drug |
| Zdroj: | Life Sciences. 39:21-28 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(86)90433-9 |
| Popis: | Methods of preventing the degradation of 3 H-methionine-enkephalin ( 3 H-ME) in human blood both at 37°C and under conditions of immediate cooling were examined. We found that, contrary to previous suggestions, use of aprotinin (with or without immediate 3ooling) was ineffective in preventing the degradation of 3 H-ME in blood. Thus, previous reports on the circulating levels of ME which relied on such procedures to stabilize the ME may have reported artifactually low values. However, we found that citric acid effectively prevents 3 H-ME breakdown in both human and rat blood. Thus, we propose the use of citric acid, mixed with blood immediately upon collection, as an effective method for the stabilization of ME in blood. |
| Databáze: | OpenAIRE |
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