Role of pomegranate extract in restoring endometrial androgen receptor expression, proliferation, and pinopodes in a rat model of polycystic ovary syndrome
| Autor: | Marwa A.A. Ibrahim, Mona Tayssir Sadek, H.E.M. Sharaf Eldin |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Uterus H&E stain Endometrium Pomegranate 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Testosterone Cell Proliferation 030219 obstetrics & reproductive medicine Estradiol Plant Extracts business.industry Luteinizing Hormone Polycystic ovary Rats Androgen receptor Ki-67 Antigen 030104 developmental biology medicine.anatomical_structure Endocrinology Receptors Androgen Androgens Female Follicle Stimulating Hormone Anatomy business Luteinizing hormone Polycystic Ovary Syndrome Hormone |
| Zdroj: | Morphologie. 106:145-154 |
| ISSN: | 1286-0115 |
| Popis: | Summary Background Polycystic ovary syndrome (PCOS) is a multifactorial hormonal disorder accompanied by impairment of endometrial function and structure. Pomegranate is recognized for its role in normalizing the female sex hormones in PCOS with little known about its effect on the accompanying endometrial histological alterations. Aim of the work To assess the possible ameliorative role of pomegranate juice extract (PJE) on endometrial injury in a rat model of PCOS. Material and methods Forty adult albino rats were equally divided into 4 groups; control, PJE-treated (400 mg/kg/day for 3 weeks), letrozole-treated (PCOS) (1 mg/kg/day for 3 weeks), and PJE & PCOS groups. Serum Follicle-stimulating hormone (FSH), Luteinizing hormone (LH), testosterone, estradiol, and tissue malondialdehyde (MDA) were assayed. Uterine samples were processed for histological staining with hematoxylin & eosin and Masson's trichrome stains, Ki67 and androgen receptor immunohistochemical staining, and scanning electron microscopy. Results PCOS group revealed a significant increase in serum FSH, LH, testosterone, estradiol, and tissue MDA. Uterine sections depicted various histological alterations in the endometrium with signs of inflammation. A significant increase in the endometrial collagen fiber content, as well as a significant upregulation in Ki67 and androgen receptor immunohistochemical expression were detected. Scanning electron microscopy showed a significant decrease in the mean number of pinopodes. Concomitant administration of PJE efficiently restored the studied biochemical, histological, and immunohistochemical parameters. Conclusion PJE ameliorated PCOS accompanying endometrial histological alterations through its antioxidant, anti-inflammatory, anti-fibrotic, anti-proliferative, and anti-androgenic effects most probably due to its polyphenols content. |
| Databáze: | OpenAIRE |
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