The Interplay between Histamine H4 Receptor and the Kidney Function: The Lesson from H4 Receptor Knockout Mice
| Autor: | Cristina Grange, Maura Gurrieri, Arianna Carolina Rosa, Roberta Cavalli, Elisa Benetti, Paolo Pollicino, Paul L. Chazot, Roberta Verta, Robin L. Thurmond, Sara Borga, Alessandro Pini |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
receptors Renal function Biochemistry Microbiology Diabetic nephropathy Basal (phylogenetics) Histamine H Internal medicine medicine Histamine H4 receptor Receptor Molecular Biology 4 Animals Aquaporin 1 Aquaporin 2 Aquaporins Diabetes Mellitus Experimental Disease Models Animal Gene Expression Regulation Histamine Hyperglycemia Kidney Mice Mice Inbred NOD Mice Knockout Receptors Histamine H4 Sodium-Hydrogen Exchanger 3 Chemistry diabetic nephropathy renal function Albumin histamine H4 receptors medicine.disease Pathophysiology QR1-502 Endocrinology Knockout mouse |
| Zdroj: | Biomolecules, Vol 11, Iss 1517, p 1517 (2021) Biomolecules, 2021, Vol.11(10) [Peer Reviewed Journal] Biomolecules Volume 11 Issue 10 |
| Popis: | Previous studies implicated the histamine H4 receptor in renal pathophysiology. The aim here is to elucidate the role of this receptor on renal function using H4 receptor knockout mice (H4R−/−). Healthy and diabetic H4R−/− mice compared to their C57BL/6J wild-type counterpart for renal function and the expression of crucial tubular proteins. H4R−/− and wild-type mice, matched for ages, showed comparable weight gain curves reaching similar median weight at the end of the study. However, H4R−/− mice displayed a higher basal glycemia. H4R−/− mice showed a lower urine 24 h outflow, and albumin-to-creatinine ratio (ACR) compared to wild-type mice. Consistently, H4R−/− mice presented a higher expression of megalin and a lower basal expression of the sodium-hydrogen exchanger (NHE)3 and aquaporin (AQP)2. According to these basal differences, diabetic H4R−/− mice developed more severe hyperglycemia and a higher 24 h urine volume, but a lower increase in ACR and decrease in urine pH were observed. These events were paralleled by a reduced NHE3 over-expression and megalin loss in diabetic H4R−/− mice. The AQP1 and AQP7 patterns were also different between H4R−/− and wild-type diabetic mice. The collected results highlight the role of the histamine H4 receptor in the control of renal reabsorption processes, particularly albumin uptake. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|