Functional Activities and Epitope Specificity of Human and Murine Antibodies against the Class 4 Outer Membrane Protein (Rmp) of Neisseria meningitidis
| Autor: | Jan Tommassen, E. Arne Høiby, Audun Aase, Ellen Namork, Alexei A. Delvig, E Wedege, Terje E. Michaelsen, Alexis Musacchio, Einar Rosenqvist, Rolf Dalseg, Jan Kolberg |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Blood Bactericidal Activity medicine.drug_class Immunoblotting Immunology Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Neisseria meningitidis Monoclonal antibody medicine.disease_cause complex mixtures Microbiology Epitope Epitopes Mice Blocking antibody medicine Animals Humans Microscopy Immunoelectron Mice Inbred BALB C biology Immunogenicity Antibodies Monoclonal bacterial infections and mycoses biology.organism_classification Antibodies Bacterial Infectious Diseases Microbial Immunity and Vaccines Bacterial Vaccines biology.protein bacteria Parasitology Neisseriaceae Antibody Bacterial outer membrane Bacterial Outer Membrane Proteins |
| Zdroj: | Infection and Immunity. 67:1267-1276 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.67.3.1267-1276.1999 |
| Popis: | Antibodies against the class 4 outer membrane protein (OMP) from Neisseria meningitidis have been purified from sera from vaccinees immunized with the Norwegian meningococcal group B outer membrane vesicle vaccine. The human sera and purified antibodies reacted strongly with the class 4 OMP in immunoblots, whereas experiments with whole bacteria showed only weak reactions, indicating that the antibodies mainly reacted with parts of the class 4 molecule that were not exposed. The purified human anti-class 4 OMP antibodies and the monoclonal antibodies (MAbs) were neither bactericidal nor opsonic against live meningococci. Three new MAbs against the class 4 OMP were generated and compared with other, previously described MAbs. Three linear epitopes in different regions of the class 4 OMP were identified by the reaction of MAbs with synthetic peptides. The MAbs showed no blocking effect on bactericidal activity of MAbs against other OMPs. However, one of the eight purified human anti-class 4 OMP antibody preparations, selected from immunoblot reactions among sera from 27 vaccinees, inhibited at high concentrations the bactericidal effect of a MAb against the class 1 OMP. However, these antibodies were not vaccine induced, as they were present also before vaccination. Therefore, this study gave no evidence that vaccination with a meningococcal outer membrane vesicle vaccine containing the class 4 OMP induces blocking antibodies. Our data indicated that the structure of class 4 OMP does not correspond to standard β-barrel structures of integral OMPs and that no substantial portion of the OmpA-like C-terminal region of this protein is located at the surface of the outer membrane. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|